Expression, purification, and characterization of the functional dimeric cytoplasmic domain of human erythrocyte band 3 in Escherichia coli

Biochemistry, Genetics and Molecular Biology

• Band 3 100%
• N-Terminus 100%
• Escherichia coli 100%
• Copurification 100%
• Plasmid 66%
• Ankyrin 66%
• Promoter Region 33%
• Fructose-Bisphosphate Aldolase 33%
• Enzyme 33%
• Binding Site 33%
• Aldolase 33%
• Protein Secondary Structure 33%
• Gene Linkage 33%
• Conformational Change 33%
• Site-Directed Mutagenesis 33%
• Amino Acid 33%
• Proteolysis 33%
• Transmembrane Protein 33%
• Membrane Protein 33%
• Hemocyte 33%

Immunology and Microbiology

• Escherichia coli 100%
• Erythrocyte 100%
• Band 3 100%
• Amino Terminal Sequence 75%
• Plasmid 50%
• Promoter Region 25%
• Protein Secondary Structure 25%
• Protein Degradation 25%
• Binding Site 25%
• Amino Acid 25%
• Conformational Transition 25%
• Site Directed Mutagenesis 25%
• Erythrocyte Membrane 25%
• Hemoglobin 25%
• Hemocyte 25%
• Skeleton 25%

Keyphrases

• Simple Steps 25%
• BL21 25%
• Expression Plasmid 25%
• Efficient Production 25%
• Crystallization 25%
• T7 Promoter 25%
• Mutagenesis Studies 25%
• Membrane Skeleton 25%
• Escherichia Coli Strains 25%
• Structure-dependent 25%