Expression, purification and characterization of codon-optimized human N-methylpurine-DNA glycosylase from Escherichia coli

Keyphrases

• Escherichia Coli 100%
• DNA Glycosylase 100%
• Codon Optimization 100%
• Expression Level 28%
• Codon 28%
• Monoclonal Antibody 14%
• High-level Expression 14%
• Kcat 14%
• Epitope 14%
• High Temperature 14%
• Purification Process 14%
• Surface Plasmon Resonance 14%
• Lipid Peroxidation 14%
• Purine 14%
• Expression System 14%
• Steady-state Kinetics 14%
• Structure Function 14%
• Optimal Activity 14%
• Hypoxanthine 14%
• Excision Repair 14%
• Isoelectric Point 14%
• DNA Repair Enzymes 14%
• Scale Purification 14%
• Equilibrium Binding Constant 14%
• Cleavage Reactions 14%
• Large-scale Production 14%
• Purification System 14%
• Poor Yield 14%
• Poor Stability 14%

Biochemistry, Genetics and Molecular Biology

• DNA Glycosylase 100%
• Escherichia coli 100%
• Codon 100%
• Copurification 100%
• Enzyme 14%
• N-Terminus 14%
• Base Excision Repair 14%
• Monoclonal Antibody 14%
• Steady State 14%
• Turnover Number 14%
• Binding Affinity 14%
• Lipid Peroxidation 14%
• DNA Ligase 14%
• Isoelectric Point 14%
• Surface Plasmon Resonance 14%
• Large Scale Production 14%
• Enzyme Kinetics 14%

Immunology and Microbiology

• Codon 100%
• Escherichia coli 100%
• Epitope 25%
• Excision Repair 25%
• Monoclonal Antibody 25%
• Steady State 25%
• Amino Terminal Sequence 25%
• DNA Repair 25%
• Binding Affinity 25%
• Lipid Peroxidation 25%
• Surface Plasmon Resonance 25%
• Turnover Number 25%
• Isoelectric Point 25%
• Repair Enzyme 25%
• Large Scale Production 25%
• Enzyme Kinetics 25%