Expression profiling using human tissues in combination with RNA amplification and microarray analysis: Assessment of Langerhans cell histiocytosis

K. L. McClain, Y. H. Cai, J. Hicks, Leif E. Peterson, X. T. Yan, S. Che, S. D. Ginsberg

    Research output: Contribution to journalShort surveypeer-review

    21 Scopus citations

    Abstract

    Advances in molecular genetics have led to sequencing of the human genome, and expression data is becoming available for many diverse tissues throughout the body, allowing for exciting hypothesis testing of critical concepts such as development, differentiation, homeostasis, and ultimately, disease pathogenesis. At present, an optimal methodology to assess gene expression is to evaluate single cells, either identified physiologically in living preparations, or by immunocytochemical or histochemical procedures in fixed cells in vitro or in vivo. Unfortunately, the quantity of RNA harvested from a single cell is not sufficient for standard RNA extraction methods. Therefore, exponential polymerase-chain reaction (PCR) based analyses, and linear RNA amplification including amplified antisense (aRNA) RNA amplification and a newly developed terminal continuation (TC) RNA amplification methodology have been used in combination with microdissection procedures such as laser capture microdissection (LCM) to enable the use of microarray platforms within individual populations of cells obtained from a variety of human tissue sources such as biopsy-derived samples {including Langerhans cell histiocytosis (LCH)} as well as postmortem brain samples for high throughput expression profiling and related downstream genetic analyses.

    Original languageEnglish (US)
    Pages (from-to)279-290
    Number of pages12
    JournalAmino Acids
    Volume28
    Issue number3
    DOIs
    StatePublished - May 2005

    Keywords

    • Cancer genomics
    • cDNA microarray
    • Molecular fingerprint
    • Postmortem human brain
    • Translational neuroscience

    ASJC Scopus subject areas

    • Clinical Biochemistry
    • Biochemistry
    • Endocrinology

    Fingerprint

    Dive into the research topics of 'Expression profiling using human tissues in combination with RNA amplification and microarray analysis: Assessment of Langerhans cell histiocytosis'. Together they form a unique fingerprint.

    Cite this