TY - JOUR
T1 - Expression profiling of human glial precursors
AU - Campanelli, James T.
AU - Sandrock, Robert W.
AU - Wheatley, Will
AU - Xue, Haipeng
AU - Zheng, Jianhua
AU - Liang, Feng
AU - Chesnut, Jonathan D.
AU - Zhan, Ming
AU - Rao, Mahendra S.
AU - Liu, Ying
N1 - Funding Information:
This work was supported by Invitrogen Corporation and Q Therapeutics Inc. The authors would like to thank Dr. Steve Goldman, Dr. Ahmet Hoke, and Cognate Inc. for providing samples. We thank all members of our laboratories for constant stimulating discussions. MSR acknowledges the contributions of Dr. S. Rao that made undertaking this project possible.
PY - 2008
Y1 - 2008
N2 - Background. We have generated gene expression databases for human glial precursors, neuronal precursors, astrocyte precursors and neural stem cells and focused on comparing the profile of glial precursors with that of other populations. Results. A total of 14 samples were analyzed. Each population, previously distinguished from each other by immunocytochemical analysis of cell surface markers, expressed genes related to their key differentiation pathways. For the glial precursor cell population, we identified 458 genes that were uniquely expressed. Expression of a subset of these individual genes was validated by RT-PCR. We also report genes encoding cell surface markers that may be useful for identification and purification of human glial precursor populations. Conclusion. We provide gene expression profile for human glial precursors. Our data suggest several signaling pathways that are important for proliferation and differentiation of human glial precursors. Such information may be utilized to further purify glial precursor populations, optimize media formulation, or study the effects of glial differentiation.
AB - Background. We have generated gene expression databases for human glial precursors, neuronal precursors, astrocyte precursors and neural stem cells and focused on comparing the profile of glial precursors with that of other populations. Results. A total of 14 samples were analyzed. Each population, previously distinguished from each other by immunocytochemical analysis of cell surface markers, expressed genes related to their key differentiation pathways. For the glial precursor cell population, we identified 458 genes that were uniquely expressed. Expression of a subset of these individual genes was validated by RT-PCR. We also report genes encoding cell surface markers that may be useful for identification and purification of human glial precursor populations. Conclusion. We provide gene expression profile for human glial precursors. Our data suggest several signaling pathways that are important for proliferation and differentiation of human glial precursors. Such information may be utilized to further purify glial precursor populations, optimize media formulation, or study the effects of glial differentiation.
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U2 - 10.1186/1471-213X-8-102
DO - 10.1186/1471-213X-8-102
M3 - Article
C2 - 18947415
AN - SCOPUS:55349108935
SN - 1471-213X
VL - 8
JO - BMC Developmental Biology
JF - BMC Developmental Biology
M1 - 102
ER -