@article{0898dcb7ba6d4a0fb0d95257f3a70760,
title = "Expression of the receptors for the C5a anaphylatoxin, interleukin-8 and FMLP by human astrocytes and microglia",
abstract = "The expression of chemotactic receptors in the central nervous system is largely unexplored. In this study, we examined human astrocytes and microglia as well as the conditionally immortalized human astrocyte cell line HSC2 for expression of the C5a-anaphylatoxin receptor (C5aR), the interleukin-8 receptor (IL-8R) and the f-Met-Leu-Phe receptor (FMLPR). Using flow cytometry, indirect immunofluorescence and RT-PCR analysis, we demonstrated that astrocytes, microglia and HSC2 cells contain specific RNA and express surface protein for all three chemotactic receptors. These are the first studies to demonstrate definitively the expression of these chemotactic receptors on astrocytes and microglia, thereby expanding the types of cells known to express chemotactic receptors. Moreover, these data suggest that these chemotactic receptors may play an important role in mediating the inflammatory response and perhaps other yet undescribed biological phenomena in the central nervous system.",
keywords = "Astrocytes, C5aR, FMLPR, Interleukin-8R, Microglia",
author = "Marie Lacy and Jennifer Jones and Whittemore, {Scott R.} and Haviland, {David L.} and Wetsel, {Rick A.} and Barnum, {Scott R.}",
note = "Funding Information: We thank Drs. Zina Moldoveanu and Jiri Mestecky for helpful suggestions and photography in the immunofluorescences tudies, and Drs. Craig Gerard and Philip Murphy for the CSa-R and IL-g-R cDNAs for use as probes in our Southern blots. We also thank Meg Mosteller-Barnum for help with RT-PCR and Marion Spell (AIDS Center, Shared Facilities Flow Cytometry Core) for the flow cytometric analysis. We thank Dr. Cheryl Harris (UAB, Department of Pathology) for critical reading of the manuscript. We also thank Les Olson and J.D. Waters of the University of Miami School of Medicine Organ Procurement Team as well as the Medical Examiners Offices of Dade, Broward, Collier, Monroe, Palm Beach and St. Lucie Counties for their assistancei n procuring spinal tissues. This work was supportedb y National Institutes of Health grants NS29719 (S.R.B.), AI25011 (R.A.W.) and NS26887 (S.R.W.) and by a grant (RG240-A-3) and pilot project (PP0399) from the National Multiple Sclerosis Society (S.R.B.) and The Miami Project to Cure Paralysis (S.R.W.). M.L. was supported by a predoctoral fellowship award (NIH T32 AI0 7051). R.A.W. was the recipient of Research Career Development Award AI00919 from the National Institutes of Health. Synthesis of oligonucleotide primers for RT-PCR was supportedb y NC1 grant CA13148 to the UAB Com-prehensiveC ancer Center.",
year = "1995",
month = aug,
doi = "10.1016/0165-5728(95)00075-D",
language = "English (US)",
volume = "61",
pages = "71--78",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier B.V.",
number = "1",
}