TY - JOUR
T1 - Expression of the peroxisome proliferator-activated receptor (PPAR) in the mouse colonic mucosa
AU - Mansén, Anethe
AU - Guardiola-Diaz, Hebé
AU - Rafter, Joseph
AU - Branting, Christina
AU - Gustafsson, Jan Åke
N1 - Funding Information:
We are grateful to Drs. Tugwood and O’Malley for providing plasmids and Dr. Karsten Kristiansen, Department of Molecular Biology, University of Odense, Denmark for a generous supply of rabbit anti-rat GST-PPARα LBD anti-serum. K. Holmberg is acknowledged for laboratory assistance and K. Grandien for helpful discussions. The study was supported by grants from the Swedish Cancer Society and the Swedish Dairies Association.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/5/24
Y1 - 1996/5/24
N2 - The peroxisome proliferator-activated receptor (PPAR), a member of the steroid nuclear receptor superfamily, has been shown to be activated by various compounds such as fibrates, thiazolidinediones, prostaglandins, and fatty acids. Here we demonstrate expression of PPAR in mouse colonic and small intestinal mocosa by Western blot analysis and immunohistochemistry, indicating a higher expression level in the differentiated colonic epithelial cells facing the intestinal lumen. Quantification of PPAR mRNA by ribonuclease protection assay revealed relatively high expression of PPARγ and Nuc1 in the colon as compared to die small intestine. In contrast, PPARα expression was higher in the small intestine as compared to the colon. These results demonstrate the presence of PPAR in the intestinal mucosa; however, the physiological roles of the various isoforms in the intestine remain to be established.
AB - The peroxisome proliferator-activated receptor (PPAR), a member of the steroid nuclear receptor superfamily, has been shown to be activated by various compounds such as fibrates, thiazolidinediones, prostaglandins, and fatty acids. Here we demonstrate expression of PPAR in mouse colonic and small intestinal mocosa by Western blot analysis and immunohistochemistry, indicating a higher expression level in the differentiated colonic epithelial cells facing the intestinal lumen. Quantification of PPAR mRNA by ribonuclease protection assay revealed relatively high expression of PPARγ and Nuc1 in the colon as compared to die small intestine. In contrast, PPARα expression was higher in the small intestine as compared to the colon. These results demonstrate the presence of PPAR in the intestinal mucosa; however, the physiological roles of the various isoforms in the intestine remain to be established.
UR - http://www.scopus.com/inward/record.url?scp=0029949369&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029949369&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0832
DO - 10.1006/bbrc.1996.0832
M3 - Article
C2 - 8651933
AN - SCOPUS:0029949369
SN - 0006-291X
VL - 222
SP - 844
EP - 851
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -