TY - JOUR
T1 - Expression of telomerase reverse transcriptase (TERT) in malignant mesotheliomas
AU - Kumaki, Fumiyuki
AU - Kawai, Toshiaki
AU - Churg, Andrew
AU - Galateau-Sallé, Françoise B.
AU - Hasleton, Philip
AU - Henderson, Douglas
AU - Roggli, Victor
AU - Travis, William D.
AU - Cagle, Philip T.
AU - Ferrans, Victor J.
PY - 2002
Y1 - 2002
N2 - To evaluate the usefulness of determinations of telomerase activity for distinguishing malignant from benign mesothelial lesions, immunohistochemical (using a rabbit polyclonal antibody and the peroxidase method; n = 68) and in situ hybridization (using sense and antisense oligonucleotide probes; n = 46) studies were made on malignant mesotheliomas (epithelioid, 39; sarcomatoid, 18, including 2 of the desmoplastic type; and biphasic, 11) and 19 benign mesothelial lesions (benign mesothelial hyperplasia, 3; and reactive pleuritis, 16). In addition, biochemical studies of telomerase activity were made in 9 of the malignant mesotheliomas. Telomerase activity was detected histochemically in all but one of the malignant mesotheliomas, but only in one (pleuritis) of the benign lesions, in which it was present only in activated lymphocytes. Antisense hybridization signals indicated the presence of telomerase mRNA mainly in the cytoplasm of the malignant cells. Sense probes gave negative results. Biochemical determinations revealed a strong telomerase activity in the 9 malignant mesotheliomas examined. This study demonstrates the usefulness of immunohistochemical staining for the evaluation of mesotheliomas. The required immunostaining can be performed using paraffin sections of formalin-fixed tissues.
AB - To evaluate the usefulness of determinations of telomerase activity for distinguishing malignant from benign mesothelial lesions, immunohistochemical (using a rabbit polyclonal antibody and the peroxidase method; n = 68) and in situ hybridization (using sense and antisense oligonucleotide probes; n = 46) studies were made on malignant mesotheliomas (epithelioid, 39; sarcomatoid, 18, including 2 of the desmoplastic type; and biphasic, 11) and 19 benign mesothelial lesions (benign mesothelial hyperplasia, 3; and reactive pleuritis, 16). In addition, biochemical studies of telomerase activity were made in 9 of the malignant mesotheliomas. Telomerase activity was detected histochemically in all but one of the malignant mesotheliomas, but only in one (pleuritis) of the benign lesions, in which it was present only in activated lymphocytes. Antisense hybridization signals indicated the presence of telomerase mRNA mainly in the cytoplasm of the malignant cells. Sense probes gave negative results. Biochemical determinations revealed a strong telomerase activity in the 9 malignant mesotheliomas examined. This study demonstrates the usefulness of immunohistochemical staining for the evaluation of mesotheliomas. The required immunostaining can be performed using paraffin sections of formalin-fixed tissues.
KW - Benign mesothelial hyperplasia
KW - Immunohistochemistry
KW - Malignant mesothelioma
KW - Pleuritis
KW - Telomerase
UR - http://www.scopus.com/inward/record.url?scp=0036187373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036187373&partnerID=8YFLogxK
U2 - 10.1097/00000478-200203000-00011
DO - 10.1097/00000478-200203000-00011
M3 - Article
C2 - 11859209
AN - SCOPUS:0036187373
SN - 0147-5185
VL - 26
SP - 365
EP - 370
JO - American Journal of Surgical Pathology
JF - American Journal of Surgical Pathology
IS - 3
ER -