Expression of tartrate‐resistant acid phosphatase in B‐CLL treated with phorbol ester or phorbol ester plus calcium ionophore

Hans G. Drexler, Malcolm K. Brenner, Suzanne M. Gignac, A. Victor Hoffbrand

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Cells from 3 patients with B-chronic lymphocytic leukaemia (B-CLL) and 1 with B-prolymphocytic leukaemia (B-PLL) were treated in vitro with the phorbol ester 12-0-tetradecanoylphorbol (TPA), the calcium ionophore A23187, or a combination of TPA and A23187. TPA induced the cells to adhere to the culture flask or to clump in dense clusters; single cells became enlarged, often with cytoplasmic elongations. Cells treated with TPA plus A23187 acquired a plasmacytoid morphology and formed regular aggregates in culture. Only TPA alone induced the expression of tartrate-resistant acid phosphatase (TRAP) as documented by isoelectric focusing on horizontal thin-layer gels. The TRAP isoenzyme was first detected after 24 h of TPA treatment; its intensity increased during further TPA exposure, being maximally expressed at 72/96 h. The results suggest that, while TPA triggers B-CLL cells to convert to hairy cell leukaemia (HCL)-type cells, the double stimulus which more closely imitates physiological activation initiates a 'normal' differentiation programme which leads to plasma cells.

Original languageEnglish (US)
Pages (from-to)250-257
Number of pages8
JournalEuropean Journal of Haematology
Volume41
Issue number3
DOIs
StatePublished - Sep 1988

Keywords

  • B‐CLL
  • TPA
  • TRAP
  • isoenzyme

ASJC Scopus subject areas

  • Hematology

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