TY - JOUR
T1 - Expression of peroxisome proliferator-activated receptor α messenger ribonucleic acid and protein in human and rat testis
AU - Schultz, Rüdiger
AU - Yan, Wei
AU - Toppari, Jorma
AU - Völkl, Alfred
AU - Gustafsson, Jan Åke
AU - Pelto-Huikko, Markku
PY - 1999
Y1 - 1999
N2 - Peroxisome proliferator-activated receptor α (PPARα), a member of the steroid hormone receptor superfamily, has been linked to lipid homeostasis and tumorigenesis in tissues with high expression of receptor protein. On the other hand, the role of PPARα in tissues with a lower expression is not well known. Here we demonstrate the localization of PPARα messenger RNA (mRNA) and protein in developing and adult rat testis. Additionally, we demonstrate the expression of PPARα protein in adult human testis. Our experiments with Northern analysis, in situ hybridization and immunocytochemistry reveal a complex distribution of PPARα in tubular and interstitial cells of both adult and developing rat testis. The overall expression is rather low but may be modified by exogenous or endogenous stimuli. An up-regulation of PPARα mRNA could be observed after stimulation with FSH. In the developing rat testis, a clear expression of PPARα mRNA was present from the first days after birth. Additionally, PPARα mRNA and protein increased toward adulthood. In adult human testis PPARα immunoreactivity (IR) was present in interstitial Leydig cells and tubular cells. In the seminiferous epithelium of adult human testis the expression of PPARα-IR could be seen in meiotic spermatocytes, spermatids and myoid peritubular cells. The findings of our study suggest that PPARα may be involved in the regulation of growth and differentiation of tubular and interstitial cells in rat and human testis.
AB - Peroxisome proliferator-activated receptor α (PPARα), a member of the steroid hormone receptor superfamily, has been linked to lipid homeostasis and tumorigenesis in tissues with high expression of receptor protein. On the other hand, the role of PPARα in tissues with a lower expression is not well known. Here we demonstrate the localization of PPARα messenger RNA (mRNA) and protein in developing and adult rat testis. Additionally, we demonstrate the expression of PPARα protein in adult human testis. Our experiments with Northern analysis, in situ hybridization and immunocytochemistry reveal a complex distribution of PPARα in tubular and interstitial cells of both adult and developing rat testis. The overall expression is rather low but may be modified by exogenous or endogenous stimuli. An up-regulation of PPARα mRNA could be observed after stimulation with FSH. In the developing rat testis, a clear expression of PPARα mRNA was present from the first days after birth. Additionally, PPARα mRNA and protein increased toward adulthood. In adult human testis PPARα immunoreactivity (IR) was present in interstitial Leydig cells and tubular cells. In the seminiferous epithelium of adult human testis the expression of PPARα-IR could be seen in meiotic spermatocytes, spermatids and myoid peritubular cells. The findings of our study suggest that PPARα may be involved in the regulation of growth and differentiation of tubular and interstitial cells in rat and human testis.
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U2 - 10.1210/endo.140.7.6858
DO - 10.1210/endo.140.7.6858
M3 - Article
C2 - 10385388
AN - SCOPUS:0033305736
SN - 0013-7227
VL - 140
SP - 2968
EP - 2975
JO - Endocrinology
JF - Endocrinology
IS - 7
ER -