Expression of neurotrophin genes in human fibroblasts: Differential regulation of the brain-derived neurotrophic factor gene

M. Cartwright, A. M. Mikheev, G. Heinrich

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) are structurally related survival and differentiation factors for distinct sets of peripheral and central neurons. We previously reported that BDNF and NGF gene expression are differentially regulated in mouse L929 fibroblasts. Here we examine expression of these three neurotrophins in human fibroblasts. Northern blots detected BDNF and NT-3 mRNAs in fibroblasts derived from lung (WI-38), calvarium and foreskin. WI-38 cells and foreskin fibroblasts expressed 1.6 kb as well as 4 kb BDNF mRNAs whereas only the smaller BDNF mRNA was detected in calvarium fibroblasts. NGF mRNA was present in foreskin and calvarium but not lung fibroblasts. In WI-38 cells serum treatment increased levels of BDNF mRNA within 2 hr. Cycloheximide did not inhibit the increase. Treatment with 12-O-tetradecanoyl phorbol-13-acetate (TPA) transiently suppressed BDNF mRNA. Treatment with both serum and TPA first stimulated and then transiently suppressed BDNF mRNA. TPA and/or serum did not significantly affect BDNF mRNA in calvarium fibroblasts. These results show that human fibroblasts derived from different tissues express and regulate neurotrophin genes differentially.

Original languageEnglish (US)
Pages (from-to)685-693
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Volume12
Issue number8
DOIs
StatePublished - Dec 1994

Keywords

  • cell signaling
  • cell specific
  • fibroblasts
  • gene expression
  • neurotrophin

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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