Expression of hsp90β messenger ribonucleic acid in patients with familial glucocorticoid resistance-Correlation to receptor status

Mikael Brönnegård, Jörgen Böös, Claude Marcus, Jaquline McGuire, Sigbritt Werner, Jan Åke Gustafsson

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We have previously shown an increased specific DNA-binding of liganded unactivated glucocorticoid receptor (GR) to the LTR-region of MMTV DNA in a patient with primary cortisol resistance and receptor thermolability indicating a defective interaction of GR with hsp90. In some patients, however, no apparent receptor abnormality was found in spite of a characteristic phenotype. mRNA expression levels of hsp90β were analysed in cultured fibroblasts from patients with known receptor defects, such as thermolability, decreased ligand binding affinity and low receptor expression levels, and from patients with a cortisol resistant phenotype but no detected receptor alteration. Fibroblasts from patients with GR defects expressed higher hsp90β mRNA levels as compared to patients with no receptor defects or to healthy controls. These data indicate that GR defects are associated with increased hsp90β mRNA levels.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume52
Issue number4
DOIs
StatePublished - Apr 1995

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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