TY - JOUR
T1 - Expression of hsp90β messenger ribonucleic acid in patients with familial glucocorticoid resistance-Correlation to receptor status
AU - Brönnegård, Mikael
AU - Böös, Jörgen
AU - Marcus, Claude
AU - McGuire, Jaquline
AU - Werner, Sigbritt
AU - Gustafsson, Jan Åke
PY - 1995/4
Y1 - 1995/4
N2 - We have previously shown an increased specific DNA-binding of liganded unactivated glucocorticoid receptor (GR) to the LTR-region of MMTV DNA in a patient with primary cortisol resistance and receptor thermolability indicating a defective interaction of GR with hsp90. In some patients, however, no apparent receptor abnormality was found in spite of a characteristic phenotype. mRNA expression levels of hsp90β were analysed in cultured fibroblasts from patients with known receptor defects, such as thermolability, decreased ligand binding affinity and low receptor expression levels, and from patients with a cortisol resistant phenotype but no detected receptor alteration. Fibroblasts from patients with GR defects expressed higher hsp90β mRNA levels as compared to patients with no receptor defects or to healthy controls. These data indicate that GR defects are associated with increased hsp90β mRNA levels.
AB - We have previously shown an increased specific DNA-binding of liganded unactivated glucocorticoid receptor (GR) to the LTR-region of MMTV DNA in a patient with primary cortisol resistance and receptor thermolability indicating a defective interaction of GR with hsp90. In some patients, however, no apparent receptor abnormality was found in spite of a characteristic phenotype. mRNA expression levels of hsp90β were analysed in cultured fibroblasts from patients with known receptor defects, such as thermolability, decreased ligand binding affinity and low receptor expression levels, and from patients with a cortisol resistant phenotype but no detected receptor alteration. Fibroblasts from patients with GR defects expressed higher hsp90β mRNA levels as compared to patients with no receptor defects or to healthy controls. These data indicate that GR defects are associated with increased hsp90β mRNA levels.
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U2 - 10.1016/0960-0760(94)00178-O
DO - 10.1016/0960-0760(94)00178-O
M3 - Article
C2 - 7734402
AN - SCOPUS:0028987019
VL - 52
SP - 345
EP - 349
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
SN - 0960-0760
IS - 4
ER -