Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo

Álan Maia Borges, Gareth David Healey, Iain Martin Sheldon

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Problem: Bacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage-associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation. Method of study: Intact bovine endometrium explants were collected using a sterile 8-mm punch biopsy and cultured ex vivo with bacteria or pathogen-associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT-mediated biotin-dUTP nick-end labelling and lactate dehydrogenase assay. Results: Intact endometrium explants accumulated IL-6, IL-1β and IL-8 in response to Gram-negative or Gram-positive bacteria, and their purified pathogen-associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium. Conclusion: This study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.

Original languageEnglish (US)
Pages (from-to)526-539
Number of pages14
JournalAmerican Journal of Reproductive Immunology
Volume67
Issue number6
DOIs
StatePublished - Jun 2012

Keywords

  • Bovine
  • Endometrium
  • Explant
  • Inflammation
  • Innate immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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