TY - JOUR
T1 - Experimental otitis media with effusion induced by nonviable Hemophilus influenzae
T2 - Cytologic and histologic study
AU - Okazaki, Nobuhiro
AU - DeMaria, Thomas F.
AU - Briggs, Bruce R.
AU - Lim, David J.
PY - 1984
Y1 - 1984
N2 - In an earlier study the authors demonstrated that formalin-killed Hemophilus influenzaeinduces serous-type middle ear effusion in chinchillas and provides an excellent model for the study of human otitis media with effusion. The present study was initiated to evaluate the morphologic and histologic changes that occur in the middle ear after injection of this organism. All of the experimental animals injected with formalin-killed H. influenzae in the present study had straw-colored serous-type effusions within four days after injection. The submucosal thickness, mononuclear cell density, and capillary permeability all increased dramatically in the experimental animals. Marked bleeding, tissue edema, and cellular infiltration in the submucosa were prominent findings after injection of the inactivated bacteria. Half of the experimental animals had histologic evidence of marked proliferation of epithelial cells resembling adhesive otitis media. These findings suggest that nonviable H. influenzae are capable of inducing severe inflammatory changes in the middle ear and may play an important role in the pathogenesis of otitis media with effusion and its sequelae.
AB - In an earlier study the authors demonstrated that formalin-killed Hemophilus influenzaeinduces serous-type middle ear effusion in chinchillas and provides an excellent model for the study of human otitis media with effusion. The present study was initiated to evaluate the morphologic and histologic changes that occur in the middle ear after injection of this organism. All of the experimental animals injected with formalin-killed H. influenzae in the present study had straw-colored serous-type effusions within four days after injection. The submucosal thickness, mononuclear cell density, and capillary permeability all increased dramatically in the experimental animals. Marked bleeding, tissue edema, and cellular infiltration in the submucosa were prominent findings after injection of the inactivated bacteria. Half of the experimental animals had histologic evidence of marked proliferation of epithelial cells resembling adhesive otitis media. These findings suggest that nonviable H. influenzae are capable of inducing severe inflammatory changes in the middle ear and may play an important role in the pathogenesis of otitis media with effusion and its sequelae.
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U2 - 10.1016/S0196-0709(84)80026-5
DO - 10.1016/S0196-0709(84)80026-5
M3 - Article
C2 - 6333829
AN - SCOPUS:0021204080
VL - 5
SP - 80
EP - 92
JO - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
JF - American Journal of Otolaryngology - Head and Neck Medicine and Surgery
SN - 0196-0709
IS - 2
ER -