Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers

Lauren E. Colbert; Molly B. El Alam; Erica J. Lynn; Julianna Bronk; Tatiana V. Karpinets; Xiaogang Wu; Bhavana V. Chapman; Travis T. Sims; Daniel Lin; Ramez Kouzy; Julie Sammouri; Greyson Biegert; Andrea Y.Delgado Medrano; Adilene Olvera; K. Jagannadha Sastry; Patricia J. Eifel; Anuja Jhingran; Lilie Lin; Lois M. Ramondetta; Andrew P. Futreal; Amir A. Jazaeri; Kathleen M. Schmeler; Jingyan Yue; Aparna Mitra; Kyoko Yoshida-Court; Jennifer A. Wargo; Travis N. Solley; Venkatesh Hegde; Sita S. Nookala; Ananta V. Yanamandra; Stephanie Dorta-Estremera; Geena Mathew; Rohit Kavukuntla; Cassidy Papso; Mustapha Ahmed-Kaddar; Minsoo Kim; Jianhua Zhang; Alexandre Reuben; Emma B. Holliday; Bruce D. Minsky; Albert C. Koong; Eugene J. Koay; Prajnan Das; Cullen M. Taniguchi; Ann Klopp

doi:10.1158/2326-6066.CIR-21-0119

Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers

Lauren E. Colbert, Molly B. El Alam, Erica J. Lynn, Julianna Bronk, Tatiana V. Karpinets, Xiaogang Wu, Bhavana V. Chapman, Travis T. Sims, Daniel Lin, Ramez Kouzy, Julie Sammouri, Greyson Biegert, Andrea Y.Delgado Medrano, Adilene Olvera, K. Jagannadha Sastry, Patricia J. Eifel, Anuja Jhingran, Lilie Lin, Lois M. Ramondetta, Andrew P. FutrealAmir A. Jazaeri, Kathleen M. Schmeler, Jingyan Yue, Aparna Mitra, Kyoko Yoshida-Court, Jennifer A. Wargo, Travis N. Solley, Venkatesh Hegde, Sita S. Nookala, Ananta V. Yanamandra, Stephanie Dorta-Estremera, Geena Mathew, Rohit Kavukuntla, Cassidy Papso, Mustapha Ahmed-Kaddar, Minsoo Kim, Jianhua Zhang, Alexandre Reuben, Emma B. Holliday, Bruce D. Minsky, Albert C. Koong, Eugene J. Koay, Prajnan Das, Cullen M. Taniguchi, Ann Klopp

Houston Methodist

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Human papillomavirus (HPV) infection causes 600,000 new cancers worldwide each year. HPV-related cancers express the oncogenic proteins E6 and E7, which could serve as tumorspecific antigens. It is not known whether immunity to E6 and E7 evolves during chemoradiotherapy or affects survival. Using T cells from 2 HPV16 patients, we conducted functional T-cell assays to identify candidate HPV-specific T cells and common T-cell receptor motifs, which we then analyzed across 86 patients with HPV-related cancers. The HPV-specific clones and E7-related T-cell receptor motifs expanded in the tumor microenvironment over the course of treatment, whereas non-HPV-specific T cells did not. In HPV16 patients, improved recurrence-free survival was associated with HPV-responsive T-cell expansion during chemoradiotherapy.

Original language	English (US)
Pages (from-to)	259-271
Number of pages	13
Journal	Cancer immunology research
Volume	10
Issue number	2
DOIs	https://doi.org/10.1158/2326-6066.CIR-21-0119
State	Published - Feb 2022

Keywords

Chemoradiotherapy
Female
Human papillomavirus 16
Humans
Oncogene Proteins, Viral
Papillomavirus E7 Proteins
Papillomavirus Infections
Prognosis
Repressor Proteins
T-Lymphocytes
Tumor Microenvironment
Uterine Cervical Neoplasms

ASJC Scopus subject areas

Medicine(all)

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10.1158/2326-6066.CIR-21-0119

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Colbert, L. E., El Alam, M. B., Lynn, E. J., Bronk, J., Karpinets, T. V., Wu, X., Chapman, B. V., Sims, T. T., Lin, D., Kouzy, R., Sammouri, J., Biegert, G., Medrano, A. Y. D., Olvera, A., Sastry, K. J., Eifel, P. J., Jhingran, A., Lin, L., Ramondetta, L. M., ... Klopp, A. (2022). Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers. Cancer immunology research, 10(2), 259-271. https://doi.org/10.1158/2326-6066.CIR-21-0119

Colbert, LE, El Alam, MB, Lynn, EJ, Bronk, J, Karpinets, TV, Wu, X, Chapman, BV, Sims, TT, Lin, D, Kouzy, R, Sammouri, J, Biegert, G, Medrano, AYD, Olvera, A, Sastry, KJ, Eifel, PJ, Jhingran, A, Lin, L, Ramondetta, LM, Futreal, AP, Jazaeri, AA, Schmeler, KM, Yue, J, Mitra, A, Yoshida-Court, K, Wargo, JA, Solley, TN, Hegde, V, Nookala, SS, Yanamandra, AV, Dorta-Estremera, S, Mathew, G, Kavukuntla, R, Papso, C, Ahmed-Kaddar, M, Kim, M, Zhang, J, Reuben, A, Holliday, EB, Minsky, BD, Koong, AC, Koay, EJ, Das, P, Taniguchi, CM & Klopp, A 2022, 'Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers', Cancer immunology research, vol. 10, no. 2, pp. 259-271. https://doi.org/10.1158/2326-6066.CIR-21-0119

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title = "Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers",

abstract = "Human papillomavirus (HPV) infection causes 600,000 new cancers worldwide each year. HPV-related cancers express the oncogenic proteins E6 and E7, which could serve as tumorspecific antigens. It is not known whether immunity to E6 and E7 evolves during chemoradiotherapy or affects survival. Using T cells from 2 HPV16 patients, we conducted functional T-cell assays to identify candidate HPV-specific T cells and common T-cell receptor motifs, which we then analyzed across 86 patients with HPV-related cancers. The HPV-specific clones and E7-related T-cell receptor motifs expanded in the tumor microenvironment over the course of treatment, whereas non-HPV-specific T cells did not. In HPV16 patients, improved recurrence-free survival was associated with HPV-responsive T-cell expansion during chemoradiotherapy.",

keywords = "Chemoradiotherapy, Female, Human papillomavirus 16, Humans, Oncogene Proteins, Viral, Papillomavirus E7 Proteins, Papillomavirus Infections, Prognosis, Repressor Proteins, T-Lymphocytes, Tumor Microenvironment, Uterine Cervical Neoplasms",

author = "Colbert, {Lauren E.} and {El Alam}, {Molly B.} and Lynn, {Erica J.} and Julianna Bronk and Karpinets, {Tatiana V.} and Xiaogang Wu and Chapman, {Bhavana V.} and Sims, {Travis T.} and Daniel Lin and Ramez Kouzy and Julie Sammouri and Greyson Biegert and Medrano, {Andrea Y.Delgado} and Adilene Olvera and Sastry, {K. Jagannadha} and Eifel, {Patricia J.} and Anuja Jhingran and Lilie Lin and Ramondetta, {Lois M.} and Futreal, {Andrew P.} and Jazaeri, {Amir A.} and Schmeler, {Kathleen M.} and Jingyan Yue and Aparna Mitra and Kyoko Yoshida-Court and Wargo, {Jennifer A.} and Solley, {Travis N.} and Venkatesh Hegde and Nookala, {Sita S.} and Yanamandra, {Ananta V.} and Stephanie Dorta-Estremera and Geena Mathew and Rohit Kavukuntla and Cassidy Papso and Mustapha Ahmed-Kaddar and Minsoo Kim and Jianhua Zhang and Alexandre Reuben and Holliday, {Emma B.} and Minsky, {Bruce D.} and Koong, {Albert C.} and Koay, {Eugene J.} and Prajnan Das and Taniguchi, {Cullen M.} and Ann Klopp",

note = "Publisher Copyright: {\textcopyright} 2022 American Association for Cancer Research.",

year = "2022",

month = feb,

doi = "10.1158/2326-6066.CIR-21-0119",

language = "English (US)",

volume = "10",

pages = "259--271",

journal = "Cancer immunology research",

issn = "2326-6066",

publisher = "American Association for Cancer Research Inc.",

number = "2",

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TY - JOUR

T1 - Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers

AU - Colbert, Lauren E.

AU - El Alam, Molly B.

AU - Lynn, Erica J.

AU - Bronk, Julianna

AU - Karpinets, Tatiana V.

AU - Wu, Xiaogang

AU - Chapman, Bhavana V.

AU - Sims, Travis T.

AU - Lin, Daniel

AU - Kouzy, Ramez

AU - Sammouri, Julie

AU - Biegert, Greyson

AU - Medrano, Andrea Y.Delgado

AU - Olvera, Adilene

AU - Sastry, K. Jagannadha

AU - Eifel, Patricia J.

AU - Jhingran, Anuja

AU - Lin, Lilie

AU - Ramondetta, Lois M.

AU - Futreal, Andrew P.

AU - Jazaeri, Amir A.

AU - Schmeler, Kathleen M.

AU - Yue, Jingyan

AU - Mitra, Aparna

AU - Yoshida-Court, Kyoko

AU - Wargo, Jennifer A.

AU - Solley, Travis N.

AU - Hegde, Venkatesh

AU - Nookala, Sita S.

AU - Yanamandra, Ananta V.

AU - Dorta-Estremera, Stephanie

AU - Mathew, Geena

AU - Kavukuntla, Rohit

AU - Papso, Cassidy

AU - Ahmed-Kaddar, Mustapha

AU - Kim, Minsoo

AU - Zhang, Jianhua

AU - Reuben, Alexandre

AU - Holliday, Emma B.

AU - Minsky, Bruce D.

AU - Koong, Albert C.

AU - Koay, Eugene J.

AU - Das, Prajnan

AU - Taniguchi, Cullen M.

AU - Klopp, Ann

PY - 2022/2

Y1 - 2022/2

N2 - Human papillomavirus (HPV) infection causes 600,000 new cancers worldwide each year. HPV-related cancers express the oncogenic proteins E6 and E7, which could serve as tumorspecific antigens. It is not known whether immunity to E6 and E7 evolves during chemoradiotherapy or affects survival. Using T cells from 2 HPV16 patients, we conducted functional T-cell assays to identify candidate HPV-specific T cells and common T-cell receptor motifs, which we then analyzed across 86 patients with HPV-related cancers. The HPV-specific clones and E7-related T-cell receptor motifs expanded in the tumor microenvironment over the course of treatment, whereas non-HPV-specific T cells did not. In HPV16 patients, improved recurrence-free survival was associated with HPV-responsive T-cell expansion during chemoradiotherapy.

AB - Human papillomavirus (HPV) infection causes 600,000 new cancers worldwide each year. HPV-related cancers express the oncogenic proteins E6 and E7, which could serve as tumorspecific antigens. It is not known whether immunity to E6 and E7 evolves during chemoradiotherapy or affects survival. Using T cells from 2 HPV16 patients, we conducted functional T-cell assays to identify candidate HPV-specific T cells and common T-cell receptor motifs, which we then analyzed across 86 patients with HPV-related cancers. The HPV-specific clones and E7-related T-cell receptor motifs expanded in the tumor microenvironment over the course of treatment, whereas non-HPV-specific T cells did not. In HPV16 patients, improved recurrence-free survival was associated with HPV-responsive T-cell expansion during chemoradiotherapy.

KW - Chemoradiotherapy

KW - Female

KW - Human papillomavirus 16

KW - Humans

KW - Oncogene Proteins, Viral

KW - Papillomavirus E7 Proteins

KW - Papillomavirus Infections

KW - Prognosis

KW - Repressor Proteins

KW - T-Lymphocytes

KW - Tumor Microenvironment

KW - Uterine Cervical Neoplasms

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UR - http://www.scopus.com/inward/citedby.url?scp=85124056415&partnerID=8YFLogxK

U2 - 10.1158/2326-6066.CIR-21-0119

DO - 10.1158/2326-6066.CIR-21-0119

M3 - Article

C2 - 35045973

AN - SCOPUS:85124056415

SN - 2326-6066

VL - 10

SP - 259

EP - 271

JO - Cancer immunology research

JF - Cancer immunology research

IS - 2

ER -

Expansion of Candidate HPV-Specific T Cells in the Tumor Microenvironment during Chemoradiotherapy Is Prognostic in HPV16 Cancers

Abstract

Keywords

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