Exosome-related lncRNAs as predictors of HCC patient survival: A prognostic model

Yuchen Hou, Zheng Yu, Nga Lei Tam, Shanzhou Huang, Chengjun Sun, Rongchang Wang, Xuzhi Zhang, Zekang Wang, Yi Ma, Xiao Shun He, Lin Wei Wu

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Objectives: Accumulating evidence suggests that long non-coding RNA (lncRNA) may affect hepatocellular carcinoma (HCC) progression. However, the mechanism remains unclear. Previous studies have shown that exosomes may promote tumor progression by transporting proteins. Our study aimed to determine the prognostic value of lncRNAs in HCC and the underlying mechanism. Methods: A dataset comprising a HCC cohort of 364 patients from The Cancer Genome Atlas (TCGA) was analyzed to identify lncRNAs with prognostic value. Co-expression and competing endogenous RNA (ceRNA) networks were constructed to investigate the mechanism of exosome-related lncRNAs. To confirm the bioinformatics analysis results, 95 pairs of clinical samples were evaluated by digoxigenin-labeled chromogenic in situ hybridization (CISH). Results: Five lncRNAs (CTD-2116N20.1, AC012074.2, RP11-538D16.2, LINC00501 and RP11-136I14.5) with significant differences were identified (P<0.001). A prognostic nomogram was constructed with a C-index of 0.701. The co-expression and ceRNA networks showed possible mechanisms for CTD-2116N20.1 and RP11-538D16.2. The CISH results confirmed that CTD-2116N20.1 and RP11-538D16.2 were correlated with a poor prognosis for HCC patients. Conclusion: Our findings provide an independent and effective prognostic model to predict the survival rate of HCC patients. RP11-538D16.2 and CTD-2116N20.1 are highlighted as important exosome-related lncRNAs.

Original languageEnglish (US)
Article numberAJTR0076252
Pages (from-to)1648-1662
Number of pages15
JournalAmerican Journal of Translational Research
Volume10
Issue number6
StatePublished - 2018

Keywords

  • Bioinformatics analysis
  • Exosome
  • Hepatocellular carcinoma (HCC)
  • Long non-coding RNA (lncRNA)
  • Nomogram

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Cancer Research

Fingerprint Dive into the research topics of 'Exosome-related lncRNAs as predictors of HCC patient survival: A prognostic model'. Together they form a unique fingerprint.

Cite this