Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs

Neal G. Copeland; Keith W. Hutchison; Nancy A. Jenkins

doi:10.1016/0092-8674(83)90419-1

Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs

Neal G. Copeland, Keith W. Hutchison, Nancy A. Jenkins

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

The dilute (d) coat color mutation of DBA/2J mice is closely associated with the site of integration of an ecotropic murine leukemia provirus. In five independent dilute revertants (d⁺) examined, reversion to wild-type coat-color correlated with the loss of most of the ecotropic proviral genome, indicating that virus integration caused the mutation. Molecular cloning and restriction enzyme analysis of the viral preintegration site as well as two independent dilute revertants, showed that exactly one long terminal repeat (LTR) remained in each revertant DNA, suggesting that virus excision occurred by homologous recombination involving the viral LTRs.

Original language	English (US)
Pages (from-to)	379-387
Number of pages	9
Journal	Cell
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(83)90419-1
State	Published - Jun 1983

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(83)90419-1

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@article{78b7763f73744dd5b1cf37be7077bdf1,

title = "Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs",

abstract = "The dilute (d) coat color mutation of DBA/2J mice is closely associated with the site of integration of an ecotropic murine leukemia provirus. In five independent dilute revertants (d+) examined, reversion to wild-type coat-color correlated with the loss of most of the ecotropic proviral genome, indicating that virus integration caused the mutation. Molecular cloning and restriction enzyme analysis of the viral preintegration site as well as two independent dilute revertants, showed that exactly one long terminal repeat (LTR) remained in each revertant DNA, suggesting that virus excision occurred by homologous recombination involving the viral LTRs.",

author = "Copeland, {Neal G.} and Hutchison, {Keith W.} and Jenkins, {Nancy A.}",

note = "Funding Information: We thank D. Bernard Amos (Duke University School of Medicine) for tissues from DBA/212D/HaAo mice, Edward Birkenmeier (The Jackson Laboratory) for the ecotropic MuLV LTR probe, Eva Either, Leslie Kozak, and Margaret Green for critical reading of this manuscript, and Eva Either for helpful suggestions concerning genetic crosses. This work was supported by Natronal Cancer lnstrtute Grant CA 32324 and American Cancer Society Grants MV-I 19 and MV-124. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1983",

month = jun,

doi = "10.1016/0092-8674(83)90419-1",

language = "English (US)",

volume = "33",

pages = "379--387",

journal = "Cell",

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T1 - Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs

AU - Copeland, Neal G.

AU - Hutchison, Keith W.

AU - Jenkins, Nancy A.

N1 - Funding Information: We thank D. Bernard Amos (Duke University School of Medicine) for tissues from DBA/212D/HaAo mice, Edward Birkenmeier (The Jackson Laboratory) for the ecotropic MuLV LTR probe, Eva Either, Leslie Kozak, and Margaret Green for critical reading of this manuscript, and Eva Either for helpful suggestions concerning genetic crosses. This work was supported by Natronal Cancer lnstrtute Grant CA 32324 and American Cancer Society Grants MV-I 19 and MV-124. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1983/6

Y1 - 1983/6

N2 - The dilute (d) coat color mutation of DBA/2J mice is closely associated with the site of integration of an ecotropic murine leukemia provirus. In five independent dilute revertants (d+) examined, reversion to wild-type coat-color correlated with the loss of most of the ecotropic proviral genome, indicating that virus integration caused the mutation. Molecular cloning and restriction enzyme analysis of the viral preintegration site as well as two independent dilute revertants, showed that exactly one long terminal repeat (LTR) remained in each revertant DNA, suggesting that virus excision occurred by homologous recombination involving the viral LTRs.

AB - The dilute (d) coat color mutation of DBA/2J mice is closely associated with the site of integration of an ecotropic murine leukemia provirus. In five independent dilute revertants (d+) examined, reversion to wild-type coat-color correlated with the loss of most of the ecotropic proviral genome, indicating that virus integration caused the mutation. Molecular cloning and restriction enzyme analysis of the viral preintegration site as well as two independent dilute revertants, showed that exactly one long terminal repeat (LTR) remained in each revertant DNA, suggesting that virus excision occurred by homologous recombination involving the viral LTRs.

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Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs

Abstract

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