TY - JOUR
T1 - Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events
AU - Sumby, Paul
AU - Porcella, Steve F.
AU - Madrigal, Andres G.
AU - Barbian, Kent D.
AU - Virtaneva, Kimmo
AU - Ricklefs, Stacy M.
AU - Sturdevant, Daniel E.
AU - Graham, Morag R.
AU - Vuopio-Varkila, Jaana
AU - Hoe, Nancy P.
AU - Musser, James M.
N1 - Funding Information:
Received 26 January 2005; accepted 1 April 2005; electronically published 29 July 2005. Data depositions: GenBank accession number for MGAS5005 genome, CP000017. Potential conflicts of interest: none reported. Financial support: National Institutes of Health (grant UO1-AI-060595). a Present affiliations: Boston University School of Medicine, Boston, Massachusetts (A.G.M.); Canadian Centre for Human and Animal Health, Winnipeg, Canada (M.R.G.). Reprints or correspondence: Dr. James M. Musser, Center for Human Bacterial Pathogenesis Research, Dept. of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030 ([email protected]).
PY - 2005/9/1
Y1 - 2005/9/1
N2 - To better understand the molecular events involved in the origin of new pathogenic bacteria, we studied the evolution of a highly virulent clone of serotype M1 group A Streptococcus (GAS). Genomic, DNA-DNA microarray, and single-nucleotide polymorphism analyses indicated that this clone evolved through a series of horizontal gene transfer events that involved (1) the acquisition of prophages encoding streptococcal pyrogenic exotoxin A and extracellular DNases and (2) the reciprocal recombination of a 36-kb chromosomal region encoding the extracellular toxins NAD+-glycohydrolase (NADase) and streptolysin O (SLO). These gene transfer events were associated with significantly increased production of SLO and NADase. Virtual identity in the 36-kb region present in contemporary serotype M1 and M12 isolates suggests that a serotype M12 strain served as the donor of this region. Multiple horizontal gene transfer events were a crucial factor in the evolutionary origin and emergence of a very abundant contemporary clone of serotype M1 GAS.
AB - To better understand the molecular events involved in the origin of new pathogenic bacteria, we studied the evolution of a highly virulent clone of serotype M1 group A Streptococcus (GAS). Genomic, DNA-DNA microarray, and single-nucleotide polymorphism analyses indicated that this clone evolved through a series of horizontal gene transfer events that involved (1) the acquisition of prophages encoding streptococcal pyrogenic exotoxin A and extracellular DNases and (2) the reciprocal recombination of a 36-kb chromosomal region encoding the extracellular toxins NAD+-glycohydrolase (NADase) and streptolysin O (SLO). These gene transfer events were associated with significantly increased production of SLO and NADase. Virtual identity in the 36-kb region present in contemporary serotype M1 and M12 isolates suggests that a serotype M12 strain served as the donor of this region. Multiple horizontal gene transfer events were a crucial factor in the evolutionary origin and emergence of a very abundant contemporary clone of serotype M1 GAS.
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U2 - 10.1086/432514
DO - 10.1086/432514
M3 - Article
C2 - 16088826
AN - SCOPUS:23944451658
SN - 0022-1899
VL - 192
SP - 771
EP - 782
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -