Evidence that the intracellular domain of FGF receptor 2IIIb affects contact of the ectodomain with two FGF7 ligands

Fumiyuki Uematsu, Jun Hyeog Jang, Mikio Kan, Fen Wang, Yongde Luo, Wallace L. McKeehan

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Models of the oligomeric FGF signaling complex, including those derived from crystal structures, vary in stoichiometry and arrangement of the three subunits comprised of heparin/heparan sulfate chains, FGFR tyrosine kinase and activating FGF. Here, using covalent affinity crosslinking of radiolabeled FGF7 to binary complexes of FGFR2IIIb and heparin, we show that two molecules of FGF7 contact each FGFR2IIIb. This supports models that propose a dimeric complex of two units with stoichiometry 1 FGF:1 FGFR in which each FGF contacts both FGFR. The bivalent FGF7 contact was dependent on the full-length amino terminus of FGF7α and the intracellular domain of FGFR2IIIb extending through the juxtamembrane domain and the β1 and β2 strands of the kinase which is required for ATP binding. We propose that the differences in crosslinking report differences in relationships among subunits in the ectodomain of the complex that are affected by the amino terminus of FGF and the FGFR intracellular domain. From this, we suggest the corollary that conformational relationships among subunits in the ectodomain are transmitted to the intracellular and ATP binding domains during activation of the complex.

Original languageEnglish (US)
Pages (from-to)791-797
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume283
Issue number4
DOIs
StatePublished - 2001

Keywords

  • Heparan sulfate
  • Oligomeric complexes
  • Transmembrane signaling
  • Tyrosine kinases

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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