Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis

Gunnar Akner; Karin Mossberg; Ann Charlotte Wikström; Karl Gösta Sundqvist; Jan Åke Gustafsson

doi:10.1016/0960-0760(91)90235-W

Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis

Gunnar Akner, Karin Mossberg, Ann Charlotte Wikström, Karl Gösta Sundqvist, Jan Åke Gustafsson

Research output: Contribution to journal › Article › peer-review

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Abstract

The cellular distribution of the glucocorticoid receptor (GR) in relation to the microtubule protein tubulin was studied in human gingival fibroblasts, using two different anti-GR antibodies of different Ig-classes, by indirect immunofluorescence immunocytology. Further studies were performed by confocal laser scanning microscopy and digital image analysis. The study focused on fluorochrome separation, optical sectioning, digital subtraction techniques and reconstruction of projections obtained using stacks of recorded transversal sections. The data presented further strengthens the notion of a structural colocalization between GR and cytoplasmic microtubules in human fibroblasts.

Original language	English (US)
Pages (from-to)	419-432
Number of pages	14
Journal	Journal of Steroid Biochemistry and Molecular Biology
Volume	39
Issue number	4 PART 1
DOIs	https://doi.org/10.1016/0960-0760(91)90235-W
State	Published - Oct 1991

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Medicine
Molecular Biology
Endocrinology
Clinical Biochemistry
Cell Biology

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Akner, G., Mossberg, K., Wikström, A. C., Sundqvist, K. G., & Gustafsson, J. Å. (1991). Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis. Journal of Steroid Biochemistry and Molecular Biology, 39(4 PART 1), 419-432. https://doi.org/10.1016/0960-0760(91)90235-W

Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis. / Akner, Gunnar; Mossberg, Karin; Wikström, Ann Charlotte et al.
In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 39, No. 4 PART 1, 10.1991, p. 419-432.

Research output: Contribution to journal › Article › peer-review

Akner, G, Mossberg, K, Wikström, AC, Sundqvist, KG & Gustafsson, JÅ 1991, 'Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis', Journal of Steroid Biochemistry and Molecular Biology, vol. 39, no. 4 PART 1, pp. 419-432. https://doi.org/10.1016/0960-0760(91)90235-W

Akner G, Mossberg K, Wikström AC, Sundqvist KG, Gustafsson JÅ. Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis. Journal of Steroid Biochemistry and Molecular Biology. 1991 Oct;39(4 PART 1):419-432. doi: 10.1016/0960-0760(91)90235-W

Akner, Gunnar ; Mossberg, Karin ; Wikström, Ann Charlotte et al. / Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis. In: Journal of Steroid Biochemistry and Molecular Biology. 1991 ; Vol. 39, No. 4 PART 1. pp. 419-432.

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title = "Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis",

abstract = "The cellular distribution of the glucocorticoid receptor (GR) in relation to the microtubule protein tubulin was studied in human gingival fibroblasts, using two different anti-GR antibodies of different Ig-classes, by indirect immunofluorescence immunocytology. Further studies were performed by confocal laser scanning microscopy and digital image analysis. The study focused on fluorochrome separation, optical sectioning, digital subtraction techniques and reconstruction of projections obtained using stacks of recorded transversal sections. The data presented further strengthens the notion of a structural colocalization between GR and cytoplasmic microtubules in human fibroblasts.",

author = "Gunnar Akner and Karin Mossberg and Wikstr{\"o}m, \{Ann Charlotte\} and Sundqvist, \{Karl G{\"o}sta\} and Gustafsson, \{Jan {\AA}ke\}",

note = "Funding Information: Acknowledgements--The valuable help from the constructive and stimulating discussions with Dr Kjell Carlsson and Professor Nils/\textasciitilde{}slund, Physics IV, The Royal Institute of Technology is greatly appreciated. This work was supported by Grants from the Swedish Medical Research Council (No. 13x-2819) and from the Swedish Cancer Society (No. 1940). Ann-Charlotte Wikstr6m is a recipient of a fellowship from the Swedish Medical Research Council.",

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doi = "10.1016/0960-0760(91)90235-W",

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Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis

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