TY - JOUR
T1 - Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis
AU - Akner, Gunnar
AU - Mossberg, Karin
AU - Wikström, Ann Charlotte
AU - Sundqvist, Karl Gösta
AU - Gustafsson, Jan Åke
N1 - Funding Information:
Acknowledgements--The valuable help from the constructive and stimulating discussions with Dr Kjell Carlsson and Professor Nils/~slund, Physics IV, The Royal Institute of Technology is greatly appreciated. This work was supported by Grants from the Swedish Medical Research Council (No. 13x-2819) and from the Swedish Cancer Society (No. 1940). Ann-Charlotte Wikstr6m is a recipient of a fellowship from the Swedish Medical Research Council.
PY - 1991/10
Y1 - 1991/10
N2 - The cellular distribution of the glucocorticoid receptor (GR) in relation to the microtubule protein tubulin was studied in human gingival fibroblasts, using two different anti-GR antibodies of different Ig-classes, by indirect immunofluorescence immunocytology. Further studies were performed by confocal laser scanning microscopy and digital image analysis. The study focused on fluorochrome separation, optical sectioning, digital subtraction techniques and reconstruction of projections obtained using stacks of recorded transversal sections. The data presented further strengthens the notion of a structural colocalization between GR and cytoplasmic microtubules in human fibroblasts.
AB - The cellular distribution of the glucocorticoid receptor (GR) in relation to the microtubule protein tubulin was studied in human gingival fibroblasts, using two different anti-GR antibodies of different Ig-classes, by indirect immunofluorescence immunocytology. Further studies were performed by confocal laser scanning microscopy and digital image analysis. The study focused on fluorochrome separation, optical sectioning, digital subtraction techniques and reconstruction of projections obtained using stacks of recorded transversal sections. The data presented further strengthens the notion of a structural colocalization between GR and cytoplasmic microtubules in human fibroblasts.
UR - http://www.scopus.com/inward/record.url?scp=0025912843&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025912843&partnerID=8YFLogxK
U2 - 10.1016/0960-0760(91)90235-W
DO - 10.1016/0960-0760(91)90235-W
M3 - Article
C2 - 1911433
AN - SCOPUS:0025912843
SN - 0960-0760
VL - 39
SP - 419
EP - 432
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 4 PART 1
ER -