Evidence for colocalization of glucocorticoid receptor with cytoplasmic microtubules in human gingival fibroblasts, using two different monoclonal anti-GR antibodies, confocal laser scanning microscopy and image analysis

Gunnar Akner, Karin Mossberg, Ann Charlotte Wikström, Karl Gösta Sundqvist, Jan Åke Gustafsson

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The cellular distribution of the glucocorticoid receptor (GR) in relation to the microtubule protein tubulin was studied in human gingival fibroblasts, using two different anti-GR antibodies of different Ig-classes, by indirect immunofluorescence immunocytology. Further studies were performed by confocal laser scanning microscopy and digital image analysis. The study focused on fluorochrome separation, optical sectioning, digital subtraction techniques and reconstruction of projections obtained using stacks of recorded transversal sections. The data presented further strengthens the notion of a structural colocalization between GR and cytoplasmic microtubules in human fibroblasts.

Original languageEnglish (US)
Pages (from-to)419-432
Number of pages14
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume39
Issue number4 PART 1
DOIs
StatePublished - Oct 1991

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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