This study was conducted to identify plasma membrane Ca2+-transporting ATPases present in rat kidney. Characterization of the cDNAs of the plasma membrane Ca2+-ATPases revealed a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of the diversity of rat renal plasma membrane Ca2+-ATPases, we used the polymerase chain reaction to detect additional gene products in rat kidney mRNA that shared these conserved regions. Sequences corresponding to three previously known rat plasma membrane Ca2+-ATPases were obtained. In addition, we found sequence corresponding to a new putative plasma membrane Ca2+-ATPase. Our results demonstrate that the rat kidney contains at least four different plasma membrane Ca2+-ATPases and the complexity of this multigene family is greater than previously thought.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 15 1992|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology