Evidence for a fourth rat isoform of the plasma membrane calcium pump in the kidney

Juan M. Gonzalez; William Dalmeida; Joel Abramowitz; Wadi N. Suki

doi:10.1016/0006-291X(92)91205-5

Evidence for a fourth rat isoform of the plasma membrane calcium pump in the kidney

Juan M. Gonzalez, William Dalmeida, Joel Abramowitz, Wadi N. Suki

Research output: Contribution to journal › Article › peer-review

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Abstract

This study was conducted to identify plasma membrane Ca²⁺-transporting ATPases present in rat kidney. Characterization of the cDNAs of the plasma membrane Ca²⁺-ATPases revealed a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of the diversity of rat renal plasma membrane Ca²⁺-ATPases, we used the polymerase chain reaction to detect additional gene products in rat kidney mRNA that shared these conserved regions. Sequences corresponding to three previously known rat plasma membrane Ca²⁺-ATPases were obtained. In addition, we found sequence corresponding to a new putative plasma membrane Ca²⁺-ATPase. Our results demonstrate that the rat kidney contains at least four different plasma membrane Ca²⁺-ATPases and the complexity of this multigene family is greater than previously thought.

Original language	English (US)
Pages (from-to)	387-393
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	184
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(92)91205-5
State	Published - Apr 15 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Evidence for a fourth rat isoform of the plasma membrane calcium pump in the kidney",

abstract = "This study was conducted to identify plasma membrane Ca2+-transporting ATPases present in rat kidney. Characterization of the cDNAs of the plasma membrane Ca2+-ATPases revealed a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of the diversity of rat renal plasma membrane Ca2+-ATPases, we used the polymerase chain reaction to detect additional gene products in rat kidney mRNA that shared these conserved regions. Sequences corresponding to three previously known rat plasma membrane Ca2+-ATPases were obtained. In addition, we found sequence corresponding to a new putative plasma membrane Ca2+-ATPase. Our results demonstrate that the rat kidney contains at least four different plasma membrane Ca2+-ATPases and the complexity of this multigene family is greater than previously thought.",

author = "Gonzalez, {Juan M.} and William Dalmeida and Joel Abramowitz and Suki, {Wadi N.}",

note = "Funding Information: %3-843 Acknowledgments: Institute and of a Diabetes, DERC This (D Di I! work -27685) estive was and pilot su I8 ported and dney feasibility Diseases in part grant by to TV ant to .N.S., J.A. DK-37543 American J.M.G. from is Heart a recipient the National Grant of # a National Kidney Foundation Fellowship and Young Investigator Awards.",

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AU - Gonzalez, Juan M.

AU - Dalmeida, William

AU - Abramowitz, Joel

AU - Suki, Wadi N.

N1 - Funding Information: %3-843 Acknowledgments: Institute and of a Diabetes, DERC This (D Di I! work -27685) estive was and pilot su I8 ported and dney feasibility Diseases in part grant by to TV ant to .N.S., J.A. DK-37543 American J.M.G. from is Heart a recipient the National Grant of # a National Kidney Foundation Fellowship and Young Investigator Awards.

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N2 - This study was conducted to identify plasma membrane Ca2+-transporting ATPases present in rat kidney. Characterization of the cDNAs of the plasma membrane Ca2+-ATPases revealed a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of the diversity of rat renal plasma membrane Ca2+-ATPases, we used the polymerase chain reaction to detect additional gene products in rat kidney mRNA that shared these conserved regions. Sequences corresponding to three previously known rat plasma membrane Ca2+-ATPases were obtained. In addition, we found sequence corresponding to a new putative plasma membrane Ca2+-ATPase. Our results demonstrate that the rat kidney contains at least four different plasma membrane Ca2+-ATPases and the complexity of this multigene family is greater than previously thought.

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Evidence for a fourth rat isoform of the plasma membrane calcium pump in the kidney

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