Evidence against a Direct Role for the Induction of c-jun Expression in the Mediation of Drug-induced Apoptosis in Human Acute Leukemia Cells

Gloria Bullock, Swapan Ray, John Reed, Toshiyuki Miyashita, Ana Maria Ibrado, Yue Huang, Kapil Bhalla

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Previous reports have demonstrated that a variety of anticancer drugs, e.g., 1-β-D-arabinofuranosylcytosine (ara-C), mitoxantrone, etoposide, camptothecin, and cisplatin, induce the expression of c-jun oncogene in leukemic cells prior to producing internucleosomal DNA fragmentation and the morphological features of apoptosis. This has led to the impression that the induction of c-jun expression may be directly involved in the molecular signaling of the final common pathway of programmed cell death or apoptosis. In the present study, we examined the role of c-jun expression in three different settings of anticancer drug-induced apoptosis in human leukemic cells. First, exposure of human myeloid leukemia HL-60 cells to high-dose ara-C for 4 h produced internucleosomal DNA fragmentation preceded by c-jun induction. However, pretreatment of HL-60 cells with staurosporine, a protein kinase C inhibitor, repressed c-jun yet enhanced DNA fragmentation and apoptosis due to ara-C. Second, in human pre-B leukemia 697/BCL-2 cells which are transfected with the cDNA of the BCL-2 oncogene and overexpress p26BCGL-2, although ara-C or miterantrone treatment caused greater c-jun induction than in the 697/neo cells, significantly reduced endonuclolytic DNA fragentation and apoptosis was observed in 697/BCL-2 cells. Finally, taxol-induced internucleosomal DNA fragmentation and morphological features of apoptosis in HL-60 cells were not associated with the induction of c-jun expression, These lines of evidence indicate that the induction of c-jun expression may not have a direct role in the molecular signaling of anticancer drug-induced apoptosis, and that the anticancer drug-induced apoptosis can occur by a mechanism that does not involve the induction of c-jun expression.

Original languageEnglish (US)
Pages (from-to)559-564
Number of pages6
JournalClinical Cancer Research
Volume1
Issue number5
StatePublished - May 1 1995

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Evidence against a Direct Role for the Induction of c-jun Expression in the Mediation of Drug-induced Apoptosis in Human Acute Leukemia Cells'. Together they form a unique fingerprint.

Cite this