Evaluation of the modified HTK solution in pancreas transplantation - An experimental model

Majid Esmaeilzadeh, Hamidreza Fonouni, Mohammad Golriz, Ali Majlesara, Gani Kuttymuratov, Frank Bergmann, Parvin Jarahian, Zahra Khazaeipour, Alireza Faridar, Morva Tahmasbi Rad, Thomas Longerich, Martha M. Gebhard, Arianeb Mehrabi

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Introduction One of the great challenges in pancreas transplantation is the ischemia reperfusion injury. It is mentioned that free oxygen and/or nitrogen radicals play a prominent role in this phase. To minimize this problem, a modified histidine-tryptophan-ketoglutarate (HTK) solution that contains modified antioxidants has been developed. Our aim was to evaluate this solution in improving the viability of the pancreas in comparison with standard HTK and University of Wisconsin (UW) solutions in a porcine model of pancreas transplantation. Materials and methods Twenty-three Landrace pigs were divided into three identical groups. After a 10-hour preservation time at 4°C, the pancreas was implanted in the organs of the recipients in a standardized manner. Serum parameters were assessed prior to and after implantation on the 1st postoperative day, 3rd postoperative day, and 7th postoperative day. Furthermore, three biopsies were taken: prior to and after reperfusion, and on Day 7 to assess the grafts. Results An analysis of serum glucose among the three groups showed no significant differences. Evaluation of the insulin levels showed no significant difference between the modified and standard HTK groups; however, differences between HTK and UW were significant (p = 0.004 in favor of UW solutions). The histopathological results showed a trend of a higher grade of rejection of pancreas tissue in the UW group compared to both HTK groups. Conclusion The modified HTK solution could preserve the pancreas for the preservation of the graft with similar results to those observed for standard solutions without any significant difference. The trend showed that the pathological finding in the UW group was not as good as that in the modified HTK and standard HTK groups.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalAsian Journal of Surgery
Volume39
Issue number2
DOIs
StatePublished - Apr 1 2016

Keywords

  • University of Wisconsin
  • histidine tryptophan ketoglutarate
  • ischemia reperfusion injury
  • pancreas transplantation

ASJC Scopus subject areas

  • Surgery

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