@article{733b3720f2374f368f849e86cbf8d616,
title = "Evaluation of the influence of fullerenol on aging and stress resistance using Caenorhabditis elegans",
abstract = "Fullerene derivatives have attracted extensive attention in biomedical fields and polyhydroxyl fullerene (fullerenol), a water-soluble fullerene derivative, is demonstrated as a powerful antioxidant. To further assess their anti-aging and anti-stress potential, we employed Caenorhabditis elegans (C.elegans) as a model organism to evaluate the effects of fullerenol on the growth, development, behavior and anti-stress ability invivo. The data show that fullerenol has no obviously toxic effect on nematodes and can delay C.elegans aging progress under normal condition. Further studies demonstrate that fullerenol attenuates endogenous levels of reactive oxygen species and provides protection to C.elegans under stress conditions by up-regulating stress-related genes in a DAF-16 depend manner and improving lifespan. In summary, our data suggest that fullerenol might be a safe and reasonable anti-aging candidate with great potential invivo.",
keywords = "Anti-aging, Caenorhabditis elegans, DAF-16, Fullerenol, Insulin/IGF-1 pathway, Lifespan",
author = "Wenshu Cong and Peng Wang and Ying Qu and Jinglong Tang and Ru Bai and Yuliang Zhao and C. Chen and Xiaolin Bi",
note = "Funding Information: This work was financially supported by the National Basic Research Program of China (973 Program) from the Ministry of Science and Technology ( 2011CB933401 , 2012CB934003 ), the National Natural Science Foundation of China ( 21320102003 , 11425520 , 31271480 ), the International Science & Technology Cooperation Program of China, Ministry of Science Technology of China ( 2013DFG32340 and 2014DFG52500 ), and Program of Changjiang Scholar and Innovative Research Team in University ( IRT13049 ). Funding Information: Wild-type Bristol N2 and all mutant strains were provided by the Caenorhabditis Genetics Center (University of Minnesota, St. Paul, MN, USA), which was funded by NIH Office of Research Infrastructure Programs (P40 OD010440). C. elegans was raised on standard nematode growth medium (NGM) plates with live Escherichia coli OP50 bacteria at 20 °C. Age-synchronous populations were prepared as previously described [25] . Synchronized L1 Larva were transferred to fresh plates with OP50 and treated with fullerenol for three days. The transgenic CF1553 (muIs84) strain containing the SOD-3::GFP-linked reporter was used to visualize SOD-3 expression, and CL2070 (dvIs70) strain containing the HSP-16.2::GFP-linked reporter was used to visualize HSP-16.2 expression. TK22 with mev-1 (succinate-CoQ oxidoreductase in the mitochondrial electron transport chain) mutation renders the nematode short lived and sensitive to oxidative stress [4] . CF1038 [daf-16 (mu86) I] is a daf-16 knockout strain. Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2015",
month = feb,
day = "1",
doi = "10.1016/j.biomaterials.2014.11.048",
language = "English (US)",
volume = "42",
pages = "78--86",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Ltd",
}