Abstract
Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization. For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).
| Original language | English (US) |
|---|---|
| Article number | 101392 |
| Journal | STAR Protocols |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 17 2022 |
Keywords
- Cell Biology
- Cell culture
- Cell isolation
- Metabolism
- Protein Biochemistry
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
- General Medicine
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