Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

Aude Angelini; Xinchun Pi; Liang Xie

doi:10.1016/j.xpro.2022.101392

Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

Aude Angelini, Xinchun Pi, Liang Xie

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization. For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).

Original language	English (US)
Article number	101392
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101392
State	Published - Jun 17 2022

Keywords

Cell Biology
Cell culture
Cell isolation
Metabolism
Protein Biochemistry

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

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10.1016/j.xpro.2022.101392

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abstract = "Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization. For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).",

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AB - Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization. For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).

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KW - Metabolism

KW - Protein Biochemistry

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Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

Abstract

Keywords

ASJC Scopus subject areas

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