TY - JOUR
T1 - Evaluation of Immune Profiles and MicroRNA Expression Profiles in Peripheral Blood Mononuclear Cells of Long-Term Stable Liver Transplant Recipients and Recipients with Acute Rejection Episodes
AU - Zhang, P.
AU - Guo, Z.
AU - Zhong, K.
AU - Li, Q.
AU - Ouyang, J.
AU - Chen, M.
AU - Hu, A.
AU - Jiao, X.
AU - Zhu, X.
AU - He, X.
N1 - Funding Information:
Funding: National High Technology Research and Development Program of China (863 Program; 2012AA021007 and 2012AA021008), Key Clinical Project from the Ministry of Health ( 2010159 ), National Natural Science Foundation of China ( 30972951 , 81102244 , 81102245 , 81170448 , 81373156 , and 81471583 ), Special Fund for Science Research by the Ministry of Health ( 201002004 and 201302009 ), Key Clinical Specialty Construction Project of the National Health and Family Planning Commission of the People's Republic of China , Research Fund for the Doctoral Program of Higher Education of China by the Ministry of Education ( 20100171110063 , 20110171120077 , and 20120171110073 ), Science and Technology Planning Key Clinical Project of Guangdong Province (2011A030400005), Project by the Division of Medical Service Management of Ministry of Health (2010), Major Sci-Tech Special Program of the Science and Technology Planning Project of Guangzhou City (2012Y2-00016), Science and Technology Planning Project of Guangzhou City (11A72060202), Key Specialty Construction Project of National Health and Family Planning Commission of People's Republic of China (2013A061401007), and Science and Technology Project of Guangdong Province (2010B031600222).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Objective This study aimed to document the difference of immunophenotypes in peripheral blood mononuclear cells (PBMCs) between long-term stable liver transplant recipients and recipients with acute rejection. We also sought to identify whether there is any correlation between microRNA (miRNA) expression profile and the differential immunoprofile in these 2 groups to establish a specific miRNA biomarker to identify potential liver transplant recipients. Methods PBMCs were isolated from 53 stable liver transplant recipients (STA group) and 15 liver transplant recipients with repeated biopsy-proven rejection episodes admitted to our hospital. Immunoprofiles were analyzed by means of flow cytometry. Analysis of miRNA expression in the PBMCs was performed by means of real-time polymerase chain reaction. Results The immune profiling analysis showed increased frequency of peripheral natural killer cells and regulatory T cells in stable liver transplant recipients compared with the acute rejection recipients and healthy volunteers (P <.05). There was no significant difference in the immune cell levels (CD19+ B cells, CD4+ T cells, and CD8+ T cells) in PBMCs among the transplant recipient groups and healthy control subjects. Three miRNAs, miR-18b, miR-340, and miR-106b, were up-regulated in the PBMCs of the STA recipients compared with recipients with acute rejection. Conclusions These results suggest that miR-18b, miR-340, and miR-106b, which regulate the expression of specific immunophenotypes, can be used as potential biomarkers to identify long-term stable liver transplant recipients from recipients with acute rejection.
AB - Objective This study aimed to document the difference of immunophenotypes in peripheral blood mononuclear cells (PBMCs) between long-term stable liver transplant recipients and recipients with acute rejection. We also sought to identify whether there is any correlation between microRNA (miRNA) expression profile and the differential immunoprofile in these 2 groups to establish a specific miRNA biomarker to identify potential liver transplant recipients. Methods PBMCs were isolated from 53 stable liver transplant recipients (STA group) and 15 liver transplant recipients with repeated biopsy-proven rejection episodes admitted to our hospital. Immunoprofiles were analyzed by means of flow cytometry. Analysis of miRNA expression in the PBMCs was performed by means of real-time polymerase chain reaction. Results The immune profiling analysis showed increased frequency of peripheral natural killer cells and regulatory T cells in stable liver transplant recipients compared with the acute rejection recipients and healthy volunteers (P <.05). There was no significant difference in the immune cell levels (CD19+ B cells, CD4+ T cells, and CD8+ T cells) in PBMCs among the transplant recipient groups and healthy control subjects. Three miRNAs, miR-18b, miR-340, and miR-106b, were up-regulated in the PBMCs of the STA recipients compared with recipients with acute rejection. Conclusions These results suggest that miR-18b, miR-340, and miR-106b, which regulate the expression of specific immunophenotypes, can be used as potential biomarkers to identify long-term stable liver transplant recipients from recipients with acute rejection.
UR - http://www.scopus.com/inward/record.url?scp=84954072657&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84954072657&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2015.10.048
DO - 10.1016/j.transproceed.2015.10.048
M3 - Article
C2 - 26707312
AN - SCOPUS:84954072657
VL - 47
SP - 2907
EP - 2915
JO - Transplantation Proceedings
JF - Transplantation Proceedings
SN - 0041-1345
IS - 10
ER -