Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing

Ryan J. Marina; Kristopher W. Brannan; Kevin D. Dong; Brian A. Yee; Gene W. Yeo

doi:10.1016/j.celrep.2020.108350

Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing

Ryan J. Marina, Kristopher W. Brannan, Kevin D. Dong, Brian A. Yee, Gene W. Yeo

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.

Original language	English (US)
Article number	108350
Journal	Cell Reports
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/j.celrep.2020.108350
State	Published - Nov 3 2020

Keywords

ADAR2
CRISPR
Cas13
Cas9
RNA editing
RNA targeting Cas9
spacerless guide RNA
transcriptome engineering

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2020.108350

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title = "Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing",

abstract = "CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.",

keywords = "ADAR2, CRISPR, Cas13, Cas9, RNA editing, RNA targeting Cas9, spacerless guide RNA, transcriptome engineering",

author = "Marina, \{Ryan J.\} and Brannan, \{Kristopher W.\} and Dong, \{Kevin D.\} and Yee, \{Brian A.\} and Yeo, \{Gene W.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

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doi = "10.1016/j.celrep.2020.108350",

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AU - Marina, Ryan J.

AU - Brannan, Kristopher W.

AU - Dong, Kevin D.

AU - Yee, Brian A.

AU - Yeo, Gene W.

PY - 2020/11/3

Y1 - 2020/11/3

N2 - CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.

AB - CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.

KW - ADAR2

KW - CRISPR

KW - Cas13

KW - Cas9

KW - RNA editing

KW - RNA targeting Cas9

KW - spacerless guide RNA

KW - transcriptome engineering

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Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing

Abstract

Keywords

ASJC Scopus subject areas

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