@article{ab29efa7839c45d084649aa1cae6003c,
title = "Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing",
abstract = "CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.",
keywords = "ADAR2, Cas13, Cas9, CRISPR, RNA editing, RNA targeting Cas9, spacerless guide RNA, transcriptome engineering",
author = "Marina, {Ryan J.} and Brannan, {Kristopher W.} and Dong, {Kevin D.} and Yee, {Brian A.} and Yeo, {Gene W.}",
note = "Funding Information: We acknowledge members of the Yeo lab, particularly Dr. David Nelles and Dr. Frederick Tan for comments and assistance in initial construct design. We thank Dr. Thorsten Stafforst of the University of T{\"u}bingen, Germany, for the gift of the W58X EGFP reporter and Dr. Patrick Hsu of the University of California, Berkeley, for the gift of the RfxCas13d-expressing plasmid used in cloning. This work was partially supported by grants from the NIH ( R01HG004659 , R01EY029166 , and R01NS103172 ) to G.W.Y. R.J.M. was supported in part by an institutional award to the UCSD Genetics Training Program from the National Institute of General Medical Sciences ( T32GM008666 ) and by a NIH/NINDS Ruth L. Kirschstein National Research Service Award ( F31NS111859 ). K.W.B. is a University of California President{\textquoteright}s Postdoctoral Fellow and is supported by a NIH/NINDS Career Transition Award ( K22NS112678 ). This publication includes data generated at the UC San Diego IGM Genomics Center using an Illumina NovaSeq 6000 instrument that was purchased with funding from a NIH SIG grant ( S10OD026929 ). Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = nov,
day = "3",
doi = "10.1016/j.celrep.2020.108350",
language = "English (US)",
volume = "33",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}