Abstract
CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.
Original language | English (US) |
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Article number | 108350 |
Journal | Cell Reports |
Volume | 33 |
Issue number | 5 |
DOIs | |
State | Published - Nov 3 2020 |
Keywords
- ADAR2
- CRISPR
- Cas13
- Cas9
- RNA editing
- RNA targeting Cas9
- spacerless guide RNA
- transcriptome engineering
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology