Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing

Ryan J. Marina, Kristopher W. Brannan, Kevin D. Dong, Brian A. Yee, Gene W. Yeo

Research output: Contribution to journalArticlepeer-review

Abstract

CRISPR-mediated transcriptome editing is an alternative strategy for genetic engineering. Marina et al. introduce a Cas9-directed method for RNA editing, which can be performed with a spacer-lacking guide RNA (gRNA). This system is comparable to other RNA-targeting Cas platforms both in on- and off-target capability.

Original languageEnglish (US)
Article number108350
JournalCell Reports
Volume33
Issue number5
DOIs
StatePublished - Nov 3 2020

Keywords

  • ADAR2
  • Cas13
  • Cas9
  • CRISPR
  • RNA editing
  • RNA targeting Cas9
  • spacerless guide RNA
  • transcriptome engineering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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