Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium

Taylor Morrisette, Katherine L. Lev, Gregory S. Canfield, Breck A. Duerkop, Razieh Kebriaei, Kyle C. Stamper, Dana Holger, Susan M. Lehman, Smaranda Willcox, Cesar A. Arias, Michael J. Rybak

Research output: Contribution to journalArticlepeer-review

Abstract

Enterococcus faecium is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. faecium strains against 4 phages, 2 phages (113 and 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, wholegenome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin (DAP) plus the phage cocktail (113 [myophage] and 9184 [siphopage]) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin-nonsusceptible E. faecium.

Original languageEnglish (US)
Article numbere01623-21
JournalAntimicrobial Agents and Chemotherapy
Volume66
Issue number1
DOIs
StatePublished - Jan 2022

Keywords

  • Bacteriophage cocktails
  • Bacteriophage-antibiotic combinations
  • Bacteriophages
  • Daptomycin
  • Enterococcus faecium

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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