Evaluation of ba1112 rhabdomyosarcoma oxygenation with microelectrodes, optical spectrophotometry, radiosensitivity, and magnetic resonance spectroscopy

H. Dirk Sostman, Sara Rockwell, Avis L. Sylvia, David Madwed, Gary Cofer, H. Cecil Charles, Rosa Negro‐Vilar, Deborah Moore

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

We studied tumor tissue oxygenation in the BA1112 rhabdomyosarcoma using microelectrode pO2 measurements, optical spectrophotometry, analyses of cell survival after irradiation, and 31P magnetic resonance spectroscopy (MRS). Studies were carried out in WAG/Rij/Y rats breathing normoxic, hypoxic, and hyperoxic gas mixtures with and without iv administration of perfluorochemical. Significant changes in tissue oxygenation and metabolic status were found when pO2 values, optical measurements of hemoglobin saturation and cytochrome a, a3 reduction-oxidation, radiation cell survival determinations, and MRS measurements of phosphometabolite ratios were obtained in rats breathing different gas mixtures. Inhalation of 100% O2 caused increases in tumor pO2, hemoglobin saturation, cytochrome a, a3 oxidation, tumor radiosensitivity, and PCr/P(i), NTP/P(i), and PDE/P(i) ratios. Such changes were augmented by pretreatment with iv perfluorochemicals. Inhalation of hypoxic gas mixtures resulted in reductions in the above parameters. These results indicate that tissue oxygenation can be manipulated reproducibly in the BA1112 rhabdomyosarcoma and suggest that 31P MRS can be used to monitor changes in tumor oxygenation in this model system.

Original languageEnglish (US)
Pages (from-to)253-267
Number of pages15
JournalMagnetic Resonance in Medicine
Volume20
Issue number2
DOIs
StatePublished - Aug 1991

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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