Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells

Joy Wolfram; Krishna Suri; Yi Huang; Roberto Molinaro; Carlotta Borsoi; Bronwyn Scott; Kathryn Boom; Donatella Paolino; Massimo Fresta; Jianghua Wang; Mauro Ferrari; Christian Celia; Haifa Shen

doi:10.3109/02652048.2013.879932

Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells

Joy Wolfram, Krishna Suri, Yi Huang, Roberto Molinaro, Carlotta Borsoi, Bronwyn Scott, Kathryn Boom, Donatella Paolino, Massimo Fresta, Jianghua Wang, Mauro Ferrari, Christian Celia, Haifa Shen

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Context: Celastrol, a natural compound derived from the herb Tripterygium wilfordii, is known to have anticancer activity, but is not soluble in water. Objective: Formation of celastrol liposomes, to avoid the use of toxic solubilising agents. Materials and methods: Two different formulations of PEGylated celastrol liposomes were fabricated. Liposomal characteristics and serum stability were determined using dynamic light scattering. Drug entrapment efficacy and drug release were measured spectrophotometrically. Cellular internalisation and anticancer activity was measured in prostate cancer cells. Results: Liposomal celastrol displayed efficient serum stability, cellular internalisation and anticancer activity, comparable to that of the free drug reconstituted in dimethyl sulfoxide. Discussion and conclusion: Liposomal celastrol can decrease the viability of prostate cancer cells, while eliminating the need for toxic solubilising agents.

Original language	English (US)
Pages (from-to)	501-507
Number of pages	7
Journal	Journal of Microencapsulation
Volume	31
Issue number	5
DOIs	https://doi.org/10.3109/02652048.2013.879932
State	Published - 2014

Keywords

Celastrol
Liposomes
Prostate cancer

ASJC Scopus subject areas

Pharmaceutical Science
Colloid and Surface Chemistry
Physical and Theoretical Chemistry
Bioengineering
Organic Chemistry

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10.3109/02652048.2013.879932

Cite this

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title = "Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells",

abstract = "Context: Celastrol, a natural compound derived from the herb Tripterygium wilfordii, is known to have anticancer activity, but is not soluble in water. Objective: Formation of celastrol liposomes, to avoid the use of toxic solubilising agents. Materials and methods: Two different formulations of PEGylated celastrol liposomes were fabricated. Liposomal characteristics and serum stability were determined using dynamic light scattering. Drug entrapment efficacy and drug release were measured spectrophotometrically. Cellular internalisation and anticancer activity was measured in prostate cancer cells. Results: Liposomal celastrol displayed efficient serum stability, cellular internalisation and anticancer activity, comparable to that of the free drug reconstituted in dimethyl sulfoxide. Discussion and conclusion: Liposomal celastrol can decrease the viability of prostate cancer cells, while eliminating the need for toxic solubilising agents.",

keywords = "Celastrol, Liposomes, Prostate cancer",

author = "Joy Wolfram and Krishna Suri and Yi Huang and Roberto Molinaro and Carlotta Borsoi and Bronwyn Scott and Kathryn Boom and Donatella Paolino and Massimo Fresta and Jianghua Wang and Mauro Ferrari and Christian Celia and Haifa Shen",

note = "Funding Information: The research was supported by funds from the Methodist Hospital Research Institute. Partial funds were acquired from: the Ernest Cockrell Jr. Distinguished Endowed Chair (M. F.), the US Department of Defense (W81XWH-09-1-0212) (M. F.), the National Institute of Health (U54CA143837, U54CA151668) (M. F.), Nylands nation Finland (J. W.), Department of Defense grant W81XWH-12-1-0414 (M. F.) and the State of Texas CPRIT grant RP121071 (M. F. and H. S.). The authors declare no conflicts of interest.",

year = "2014",

doi = "10.3109/02652048.2013.879932",

language = "English (US)",

volume = "31",

pages = "501--507",

journal = "Journal of Microencapsulation",

issn = "0265-2048",

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TY - JOUR

T1 - Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells

AU - Wolfram, Joy

AU - Suri, Krishna

AU - Huang, Yi

AU - Molinaro, Roberto

AU - Borsoi, Carlotta

AU - Scott, Bronwyn

AU - Boom, Kathryn

AU - Paolino, Donatella

AU - Fresta, Massimo

AU - Wang, Jianghua

AU - Ferrari, Mauro

AU - Celia, Christian

AU - Shen, Haifa

N1 - Funding Information: The research was supported by funds from the Methodist Hospital Research Institute. Partial funds were acquired from: the Ernest Cockrell Jr. Distinguished Endowed Chair (M. F.), the US Department of Defense (W81XWH-09-1-0212) (M. F.), the National Institute of Health (U54CA143837, U54CA151668) (M. F.), Nylands nation Finland (J. W.), Department of Defense grant W81XWH-12-1-0414 (M. F.) and the State of Texas CPRIT grant RP121071 (M. F. and H. S.). The authors declare no conflicts of interest.

PY - 2014

Y1 - 2014

N2 - Context: Celastrol, a natural compound derived from the herb Tripterygium wilfordii, is known to have anticancer activity, but is not soluble in water. Objective: Formation of celastrol liposomes, to avoid the use of toxic solubilising agents. Materials and methods: Two different formulations of PEGylated celastrol liposomes were fabricated. Liposomal characteristics and serum stability were determined using dynamic light scattering. Drug entrapment efficacy and drug release were measured spectrophotometrically. Cellular internalisation and anticancer activity was measured in prostate cancer cells. Results: Liposomal celastrol displayed efficient serum stability, cellular internalisation and anticancer activity, comparable to that of the free drug reconstituted in dimethyl sulfoxide. Discussion and conclusion: Liposomal celastrol can decrease the viability of prostate cancer cells, while eliminating the need for toxic solubilising agents.

AB - Context: Celastrol, a natural compound derived from the herb Tripterygium wilfordii, is known to have anticancer activity, but is not soluble in water. Objective: Formation of celastrol liposomes, to avoid the use of toxic solubilising agents. Materials and methods: Two different formulations of PEGylated celastrol liposomes were fabricated. Liposomal characteristics and serum stability were determined using dynamic light scattering. Drug entrapment efficacy and drug release were measured spectrophotometrically. Cellular internalisation and anticancer activity was measured in prostate cancer cells. Results: Liposomal celastrol displayed efficient serum stability, cellular internalisation and anticancer activity, comparable to that of the free drug reconstituted in dimethyl sulfoxide. Discussion and conclusion: Liposomal celastrol can decrease the viability of prostate cancer cells, while eliminating the need for toxic solubilising agents.

KW - Celastrol

KW - Liposomes

KW - Prostate cancer

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Evaluation of anticancer activity of celastrol liposomes in prostate cancer cells

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