Estrogen receptor β mRNA in colon cancer cells: Growth effects of estrogen and genistein

Naoya Arai, Anders Ström, Joseph J. Rafter, Jan Åke Gustafsson

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

Knowledge regarding the expression of the recently cloned estrogen receptor β (ERβ) in colonic mucosa is limited. In this study, we demonstrated that five human colon cancer cell lines, HT29, Colo320, Lovo, SW480, and HCT116, expressed ERβ mRNA, but lacked ERα mRNA. Results from a cell growth assay demonstrated that these colon cancer cells were not influenced by estrogen, while genistein possessed slight growth inhibitory effects on HT29, Colo320 and Lovo cells at 10 μM, at which concentration is stimulated the growth of ERα-positive human breast cancer MCF-7 cells. Tamoxifen inhibited the growth of HT29 and Colo320 cells, dose-dependently, as well as MCF-7 cells. A transfected reporter plasmid containing a vitellogenin estrogen response element could be activated by estradiol in Colo320 cells. Taken together with previous reports, these data suggest that ERα and ERP may have different biological functions in colon cells. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)425-431
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number2
DOIs
StatePublished - Apr 13 2000

Keywords

  • Colon cancer
  • Colonic cells
  • Estrogen receptor β
  • Estrogens
  • Genistein
  • Phytoestrogens

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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