TY - JOUR
T1 - Estrogen receptor β mRNA in colon cancer cells
T2 - Growth effects of estrogen and genistein
AU - Arai, Naoya
AU - Ström, Anders
AU - Rafter, Joseph J.
AU - Gustafsson, Jan Åke
N1 - Funding Information:
We thank Kristina Holmberg for valuable technical assistance. This study was supported by grants from the Swedish Cancer Society, the Swedish Institute, and Japanese Society for Promotion of Science.
PY - 2000/4/13
Y1 - 2000/4/13
N2 - Knowledge regarding the expression of the recently cloned estrogen receptor β (ERβ) in colonic mucosa is limited. In this study, we demonstrated that five human colon cancer cell lines, HT29, Colo320, Lovo, SW480, and HCT116, expressed ERβ mRNA, but lacked ERα mRNA. Results from a cell growth assay demonstrated that these colon cancer cells were not influenced by estrogen, while genistein possessed slight growth inhibitory effects on HT29, Colo320 and Lovo cells at 10 μM, at which concentration is stimulated the growth of ERα-positive human breast cancer MCF-7 cells. Tamoxifen inhibited the growth of HT29 and Colo320 cells, dose-dependently, as well as MCF-7 cells. A transfected reporter plasmid containing a vitellogenin estrogen response element could be activated by estradiol in Colo320 cells. Taken together with previous reports, these data suggest that ERα and ERP may have different biological functions in colon cells. (C) 2000 Academic Press.
AB - Knowledge regarding the expression of the recently cloned estrogen receptor β (ERβ) in colonic mucosa is limited. In this study, we demonstrated that five human colon cancer cell lines, HT29, Colo320, Lovo, SW480, and HCT116, expressed ERβ mRNA, but lacked ERα mRNA. Results from a cell growth assay demonstrated that these colon cancer cells were not influenced by estrogen, while genistein possessed slight growth inhibitory effects on HT29, Colo320 and Lovo cells at 10 μM, at which concentration is stimulated the growth of ERα-positive human breast cancer MCF-7 cells. Tamoxifen inhibited the growth of HT29 and Colo320 cells, dose-dependently, as well as MCF-7 cells. A transfected reporter plasmid containing a vitellogenin estrogen response element could be activated by estradiol in Colo320 cells. Taken together with previous reports, these data suggest that ERα and ERP may have different biological functions in colon cells. (C) 2000 Academic Press.
KW - Colon cancer
KW - Colonic cells
KW - Estrogen receptor β
KW - Estrogens
KW - Genistein
KW - Phytoestrogens
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U2 - 10.1006/bbrc.2000.2444
DO - 10.1006/bbrc.2000.2444
M3 - Article
C2 - 10753641
AN - SCOPUS:0034643293
SN - 0006-291X
VL - 270
SP - 425
EP - 431
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -