Estrogen receptor-β messenger ribonucleic acid ontogeny in the prostate of normal and neonatally estrogenized rats

Gail S. Prins, Michael Marmer, Carl Woodham, William Chang, George Kuiper, Jan Åke Gustafsson, Lynn Birch

Research output: Contribution to journalArticle

150 Scopus citations

Abstract

Neonatal exposure to estrogens permanently alters rat prostate growth and epithelial differentiation leading to prostatic dysplasia on aging. The effects are lobe-specific, with the greatest response observed in the ventral lobe. Recently, a novel estrogen receptor (ER) complementary DNA was cloned from the rat prostate and termed ER-β (ERβ) due to its high homology with the classical ERα. The protein possesses high affinity for 17β-estradiol, indicating that ERβ is an alternate molecule for mediating estrogenic effects. Importantly, ERβ messenger RNA (mRNA) was localized to rat prostatic epithelial cells, which contrasts with the stromal localization of ERα in the rat prostate. The present study was undertaken to determine the ontogeny of ERβ mRNA expression in the rat prostate lobes and to examine the effects of early estrogen exposure on prostatic ERβ expression. Male rat pups were given 25 μg estradiol or oil on days 1, 3, and 5; were killed on day 1, 3 (oils only), 6, 10, 30, or 90; and prostate lobes were frozen. Longitudinal sections were processed for in situ hybridization using an 35S-labeled antisense mRNA probe corresponding to a 400-bp EcoRI-AccI fragment in the 5'untranslated region of rat ERβ complementary DNA. Image analysis was used to quantitate silver grains. In addition, total RNA was isolated from the ventral prostate (VP) and used for semiquantitative RT- PCR. Results from in situ hybridization revealed that at birth, ERβ was equivalently expressed at low levels in both mesenchymal and epithelial cells in oil-treated rats. From day 1 onwards, expression in all stromal cells slowly and significantly declined, so that in the control adult prostate, stromal ERβ mRNA was slightly above background. In the oil-treated control rats, epithelial ERβ mRNA increased to moderate levels between days 6-10 in the VP and days 10-15 in the dorsal and lateral lobes as cells began differentiation and ducts lumenized. A further significant increase in ERβ message was observed at day 30, which indicates that full epithelial ERβ expression may require the completion of functional differentiation. By day 90, expression levels were maximal and similar between the lobes. RT-PCR substantiated this developmental increase in ERβ between days 1-90. Neonatal exposure to estrogens did not have an immediate effect on prostatic ERβ mRNA levels as determined by in situ hybridization and RT-PCR. However, the marked increase in epithelial cell expression at day 30 observed in the control VP was dampened in the VP of animals exposed neonatally to estrogens. By day 90, the VP of estrogenized rats possessed low ERβ message levels compared with the high expression in oil controls. In contrast, the dorsal and lateral lobes of neonatally estrogenized rats possessed high levels of ERβ mRNA at day 90, equivalent to controls. The present data demonstrate that ERβ mRNA expression in the rat prostate is developmentally regulated, and that neonatal estrogen can affect this expression in the adult VP. Because the effect of neonatal estrogens was not immediate, the data imply that early estrogen exposure may not directly autoregulate ERβ expression, and suggests that the adult effects on ERβ mRNA expression may be indirect. The differences in ERβ mRNA imprinting in the separate lobes may account for or reflect the lobe-specific neonatal estrogen imprints previously observed in the rat prostate.

Original languageEnglish (US)
Pages (from-to)874-883
Number of pages10
JournalEndocrinology
Volume139
Issue number3
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Endocrinology

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