TY - JOUR
T1 - Estrogen receptor β is expressed in human stomach adenocarcinoma
AU - Matsuyama, Satoru
AU - Ohkura, Yasuo
AU - Eguchi, Hidetaka
AU - Kobayashi, Yasuhito
AU - Akagi, Kiwamu
AU - Uchida, Kenji
AU - Nakachi, Kei
AU - Gustafsson, Jan Åke
AU - Hayashi, Shin Ichi
N1 - Funding Information:
Acknowledgements This work was supported by the following Grants-in-Aid: for Cancer Research from the Ministry of Education, Culture, Science, Sports and Technology, and for Health, Labor and Welfare Programs and the Foundation for the Promotion of Cancer Research, and by 2nd-Term Comprehensive 10-Year Strategy for Cancer Control, and for Comprehensive Research on Aging and Health from Ministry of Health, Labor and Welfare, Japan; from the Smoking Research Foundation of Japan, as well as by a grant from the Swedish Cancer Fund.
PY - 2002
Y1 - 2002
N2 - Purpose: In stomach adenocarcinoma, the role of the hormonal receptor, estrogen receptor (ER), has been controversial. Recently, a new estrogen receptor, called estrogen receptor β (ERβ), was found to be expressed in various tissues including normal gastrointestinal tract. In this paper, the expression of ERβ in stomach adenocarcinomas has been investigated for the first time, specifically in signet ring cell adenocarcinomas, together with surrounding non-cancerous tissues. Methods: By immunohistochemistry the expression of ERα and β was studied in 29 stomach adenocarcinomas, ten signet ring cell adenocarcinomas, and 19 other adenocarcinomas. Western blotting was performed to examine the immunohistochemical result. Statistical studies (Student's t test and x2-test) explored the relation between the immunohistochemical result and clinicopathological characteristics. Results: All 29 adenocarcinomas, including the signet ring cell ones, demonstrated clear ERβ nucleus staining. Lymphocytes, venous endothelial cells, smooth muscle, and non-cancerous stomach glands also showed strong ERβ staining, while no staining was observed in the immunohistochemistry of ERα. Western blotting showed equivalent ERβ protein levels in cancerous and non-cancerous tissues, which was consistent with the results of immunohistochemical staining. Among signet ring cell adenocarcinomas of the stomach, cytoplasm were stained in addition to nuclei, specifically in patients under the age of 40 years. Conclusions: Our results imply that the effects of estrogen in stomach cancer, as well as those in normal stomach, may be mediated by ERβ, and that the role of ERβ may differ by the subtype of stomach adenocarcinoma - specifically signet ring cell adenocarcinomas and other ones - although large scale samples are needed to confirm these findings.
AB - Purpose: In stomach adenocarcinoma, the role of the hormonal receptor, estrogen receptor (ER), has been controversial. Recently, a new estrogen receptor, called estrogen receptor β (ERβ), was found to be expressed in various tissues including normal gastrointestinal tract. In this paper, the expression of ERβ in stomach adenocarcinomas has been investigated for the first time, specifically in signet ring cell adenocarcinomas, together with surrounding non-cancerous tissues. Methods: By immunohistochemistry the expression of ERα and β was studied in 29 stomach adenocarcinomas, ten signet ring cell adenocarcinomas, and 19 other adenocarcinomas. Western blotting was performed to examine the immunohistochemical result. Statistical studies (Student's t test and x2-test) explored the relation between the immunohistochemical result and clinicopathological characteristics. Results: All 29 adenocarcinomas, including the signet ring cell ones, demonstrated clear ERβ nucleus staining. Lymphocytes, venous endothelial cells, smooth muscle, and non-cancerous stomach glands also showed strong ERβ staining, while no staining was observed in the immunohistochemistry of ERα. Western blotting showed equivalent ERβ protein levels in cancerous and non-cancerous tissues, which was consistent with the results of immunohistochemical staining. Among signet ring cell adenocarcinomas of the stomach, cytoplasm were stained in addition to nuclei, specifically in patients under the age of 40 years. Conclusions: Our results imply that the effects of estrogen in stomach cancer, as well as those in normal stomach, may be mediated by ERβ, and that the role of ERβ may differ by the subtype of stomach adenocarcinoma - specifically signet ring cell adenocarcinomas and other ones - although large scale samples are needed to confirm these findings.
KW - ERβ
KW - Signet ring cell adenocarcinoma
KW - Stomach adenocarcinoma
UR - http://www.scopus.com/inward/record.url?scp=0036258102&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036258102&partnerID=8YFLogxK
U2 - 10.1007/s00432-002-0336-3
DO - 10.1007/s00432-002-0336-3
M3 - Article
C2 - 12073050
AN - SCOPUS:0036258102
VL - 128
SP - 319
EP - 324
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
SN - 0171-5216
IS - 6
ER -