Estrogen receptor-α knockout mice

Per Antonson, Patricia Humire, Jan-Ake Gustafsson

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Tissue specific knockout mice are valuable tools to study gene function in vivo. The method uses the Cre/loxP system in which loxP sites are cloned into the genome surrounding one or more exons of a gene and the targeted exon(s) are deleted when the Cre enzyme is expressed. Mouse lines that are prepared for the generation of knockout ERα mice have been developed independently by many research groups and the number of available transgenic mouse lines that express Cre under tissue specific promoters is large. Here, we describe how tissue specific ERα knockout mice are generated.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages425-430
Number of pages6
Volume1366
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1366
ISSN (Print)10643745

Keywords

  • Cre enzyme
  • Estrogen receptor
  • Floxed
  • Gene targeting
  • Genotyping
  • Knockout mice
  • LoxP
  • Tissue specific
  • Transgenic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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