Estrogen enhances induction of cytochrome P-4501A1 by 2,3,7, 8-tetrachlorodibenzo-p-dioxin in liver of female Long-Evans rats.

S. Sarkar, N. R. Jana, J. Yonemoto, C. Tohyama, H. Sone

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A human carcinogen, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces liver tumors in female rats. In this study, we examined the effects of estrogen on an arylhydrocarbon receptor (AhR)-responsible protein, CYP1A1 expression induced by TCDD in female rat livers. The induction of CYP1A1 by a dose of 300 ng TCDD/kg and its resultant enzymatic activity were significantly enhanced by 5 microg 17beta-estradiol/kg body weight treatment to both intact and ovariectomized rats. Immunoblot analysis showed a increase in nuclear AhR due to estrogen, TCDD or both, suggesting that estrogen is involved in the activation of CYP1A1 gene after the formation of AhR-TCDD complex.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalInternational Journal of Oncology
Volume16
Issue number1
DOIs
StatePublished - Jan 2000

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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