Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture

L. M. Garcia-Segura; G. Olmos; R. J. Robbins; P. Hernandez; J. H. Meyer; F. Naftolin

doi:10.1016/0006-8993(89)91113-X

Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture

L. M. Garcia-Segura, G. Olmos, R. J. Robbins, P. Hernandez, J. H. Meyer, F. Naftolin

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Exo-endocytotic images and intramembrane particles were quantitatively assessed in freeze-fracture replicas from the plasma membrane of dispersed fetal rat cortical neurons (day 16 gestation) grown for 24 days in culture. The addition of 10^-10 M 17β-estradiol to the culture medium resulted in a significant increase in the numerical density of exo-endocytotic images within 1 min. A further increase of the number of exo-endocytotic images associated to a significant decrease in the number of intramembrane particles was observed in cells exposed for 10 min to 17β-estradiol. Similar results were observed when the cells were exposed to 17β-estradiol for 17 days. No effects on exo-endocytotic images and intramembrane particles were observed when 17α-estradiol was added, instead of 17β-estradiol, to the cultures. These results indicate that physiological levels of 17β-estradiol can have rapid effects upon the ultrastructure of the neuronal membrane of developing cerebrocortical neurons.

Original language	English (US)
Pages (from-to)	339-343
Number of pages	5
Journal	Brain Research
Volume	498
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(89)91113-X
State	Published - Oct 2 1989

Keywords

Cell culture
Cerebral cortex
Development
Estradiol
Freeze-fracture
Neuronal membrane
Plasma membrane

ASJC Scopus subject areas

Neuroscience(all)
Clinical Neurology
Molecular Biology
Developmental Biology

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10.1016/0006-8993(89)91113-X

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title = "Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture",

abstract = "Exo-endocytotic images and intramembrane particles were quantitatively assessed in freeze-fracture replicas from the plasma membrane of dispersed fetal rat cortical neurons (day 16 gestation) grown for 24 days in culture. The addition of 10-10 M 17β-estradiol to the culture medium resulted in a significant increase in the numerical density of exo-endocytotic images within 1 min. A further increase of the number of exo-endocytotic images associated to a significant decrease in the number of intramembrane particles was observed in cells exposed for 10 min to 17β-estradiol. Similar results were observed when the cells were exposed to 17β-estradiol for 17 days. No effects on exo-endocytotic images and intramembrane particles were observed when 17α-estradiol was added, instead of 17β-estradiol, to the cultures. These results indicate that physiological levels of 17β-estradiol can have rapid effects upon the ultrastructure of the neuronal membrane of developing cerebrocortical neurons.",

keywords = "Cell culture, Cerebral cortex, Development, Estradiol, Freeze-fracture, Neuronal membrane, Plasma membrane",

author = "Garcia-Segura, {L. M.} and G. Olmos and Robbins, {R. J.} and P. Hernandez and Meyer, {J. H.} and F. Naftolin",

note = "Funding Information: Supported by CSIC/CAICYT (ID751) and NIH (HD13587, NS06208) grants and by a fellowship to G.O. (FISS). We thank E. Valero and J. Sancho for technical assistance.",

year = "1989",

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doi = "10.1016/0006-8993(89)91113-X",

language = "English (US)",

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T1 - Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture

AU - Garcia-Segura, L. M.

AU - Olmos, G.

AU - Robbins, R. J.

AU - Hernandez, P.

AU - Meyer, J. H.

AU - Naftolin, F.

N1 - Funding Information: Supported by CSIC/CAICYT (ID751) and NIH (HD13587, NS06208) grants and by a fellowship to G.O. (FISS). We thank E. Valero and J. Sancho for technical assistance.

PY - 1989/10/2

Y1 - 1989/10/2

N2 - Exo-endocytotic images and intramembrane particles were quantitatively assessed in freeze-fracture replicas from the plasma membrane of dispersed fetal rat cortical neurons (day 16 gestation) grown for 24 days in culture. The addition of 10-10 M 17β-estradiol to the culture medium resulted in a significant increase in the numerical density of exo-endocytotic images within 1 min. A further increase of the number of exo-endocytotic images associated to a significant decrease in the number of intramembrane particles was observed in cells exposed for 10 min to 17β-estradiol. Similar results were observed when the cells were exposed to 17β-estradiol for 17 days. No effects on exo-endocytotic images and intramembrane particles were observed when 17α-estradiol was added, instead of 17β-estradiol, to the cultures. These results indicate that physiological levels of 17β-estradiol can have rapid effects upon the ultrastructure of the neuronal membrane of developing cerebrocortical neurons.

AB - Exo-endocytotic images and intramembrane particles were quantitatively assessed in freeze-fracture replicas from the plasma membrane of dispersed fetal rat cortical neurons (day 16 gestation) grown for 24 days in culture. The addition of 10-10 M 17β-estradiol to the culture medium resulted in a significant increase in the numerical density of exo-endocytotic images within 1 min. A further increase of the number of exo-endocytotic images associated to a significant decrease in the number of intramembrane particles was observed in cells exposed for 10 min to 17β-estradiol. Similar results were observed when the cells were exposed to 17β-estradiol for 17 days. No effects on exo-endocytotic images and intramembrane particles were observed when 17α-estradiol was added, instead of 17β-estradiol, to the cultures. These results indicate that physiological levels of 17β-estradiol can have rapid effects upon the ultrastructure of the neuronal membrane of developing cerebrocortical neurons.

KW - Cell culture

KW - Cerebral cortex

KW - Development

KW - Estradiol

KW - Freeze-fracture

KW - Neuronal membrane

KW - Plasma membrane

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Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture

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