Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture

L. M. Garcia-Segura, G. Olmos, R. J. Robbins, P. Hernandez, J. H. Meyer, F. Naftolin

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Exo-endocytotic images and intramembrane particles were quantitatively assessed in freeze-fracture replicas from the plasma membrane of dispersed fetal rat cortical neurons (day 16 gestation) grown for 24 days in culture. The addition of 10-10 M 17β-estradiol to the culture medium resulted in a significant increase in the numerical density of exo-endocytotic images within 1 min. A further increase of the number of exo-endocytotic images associated to a significant decrease in the number of intramembrane particles was observed in cells exposed for 10 min to 17β-estradiol. Similar results were observed when the cells were exposed to 17β-estradiol for 17 days. No effects on exo-endocytotic images and intramembrane particles were observed when 17α-estradiol was added, instead of 17β-estradiol, to the cultures. These results indicate that physiological levels of 17β-estradiol can have rapid effects upon the ultrastructure of the neuronal membrane of developing cerebrocortical neurons.

Original languageEnglish (US)
Pages (from-to)339-343
Number of pages5
JournalBrain Research
Volume498
Issue number2
DOIs
StatePublished - Oct 2 1989

Keywords

  • Cell culture
  • Cerebral cortex
  • Development
  • Estradiol
  • Freeze-fracture
  • Neuronal membrane
  • Plasma membrane

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture'. Together they form a unique fingerprint.

Cite this