Essential role of the tyrosine kinase substrate phospholipase C-γ1 in mammalian growth and development

Qun Sheng Ji, Glenn E. Winnier, Kevin D. Niswender, Debra Horstman, Ron Wisdom, Mark A. Magnuson, Graham Carpenter

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

The activation of many tyrosine kinases leads to the phosphorylation and activation of phospholipase C-γ1 (PLC-γ1). To examine the biological function of this protein, homologous recombination has been used to selectively disrupt the Plcg1 gene in mice. Homozygous disruption of Plcg1 results in embryonic lethality at approximately embryonic day (E) 9.0. Histological analysis indicates that Plcg1 (-/-) embryos appear normal at E 8.5 but fail to continue normal development and growth beyond E 8.5-E9.0. These results clearly demonstrate that PLC-γ1 with, by inference, its capacity to mobilize second messenger molecules is an essential signal transducing molecule whose absence is not compensated by other signaling pathways or other genes encoding PLC isozymes.

Original languageEnglish (US)
Pages (from-to)2999-3003
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number7
DOIs
StatePublished - Apr 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • General

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