TY - JOUR
T1 - Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform
AU - Hu, Che Ming J.
AU - Zhang, Li
AU - Aryal, Santosh
AU - Cheung, Connie
AU - Fang, Ronnie H.
AU - Zhang, Liangfang
PY - 2011/7/5
Y1 - 2011/7/5
N2 - Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nano-particles revealed superior circulation half-life by the erythrocytemimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.
AB - Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nano-particles revealed superior circulation half-life by the erythrocytemimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.
KW - Biomimetic nanoparticle
KW - Drug delivery
KW - Long circulation
KW - Red blood cell membrane
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U2 - 10.1073/pnas.1106634108
DO - 10.1073/pnas.1106634108
M3 - Article
C2 - 21690347
AN - SCOPUS:79960583505
VL - 108
SP - 10980
EP - 10985
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 27
ER -