ERα expression in T lymphocytes is dispensable for estrogenic effects in bone

K. L. Gustafsson; K. H. Nilsson; H. H. Farman; A. Andersson; V. Lionikaite; P. Henning; J. Wu; S. H. Windahl; U. Islander; S. Movérare-Skrtic; K. Sjögren; H. Carlsten; J. Gustafsson; C. Ohlsson; M. K. Lagerquist

doi:10.1530/JOE-18-0183

ERα expression in T lymphocytes is dispensable for estrogenic effects in bone

K. L. Gustafsson, K. H. Nilsson, H. H. Farman, A. Andersson, V. Lionikaite, P. Henning, J. Wu, S. H. Windahl, U. Islander, S. Movérare-Skrtic, K. Sjögren, H. Carlsten, J. Gustafsson, C. Ohlsson, M. K. Lagerquist

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Estrogen treatment has positive effects on the skeleton, and we have shown that estrogen receptor alpha (ERα) expression in cells of hematopoietic origin contributes to a normal estrogen treatment response in bone tissue. T lymphocytes are implicated in the estrogenic regulation of bone mass, but it is not known whether T lymphocytes are direct estrogen target cells. Therefore, the aim of this study was to determine the importance of ERα expression in T lymphocytes for the estrogenic regulation of the skeleton using female mice lacking ERα expression specifically in T lymphocytes (Lck-ERα^-/-) and ERα^flox/flox littermate (control) mice. Deletion of ERα expression in T lymphocytes did not affect bone mineral density (BMD) in sham-operated Lck-ERα^-/- compared to control mice, and ovariectomy (ovx) resulted in a similar decrease in BMD in control and Lck-ERα^-/- mice compared to sham-operated mice. Furthermore, estrogen treatment of ovx Lck-ERα^-/- led to an increased BMD that was indistinguishable from the increase seen after estrogen treatment of ovx control mice. Detailed analysis of both the appendicular (femur) and axial (vertebrae) skeleton showed that both trabecular and cortical bone parameters responded to a similar extent regardless of the presence of ERα in T lymphocytes. In conclusion, ERα expression in T lymphocytes is dispensable for normal estrogenic regulation of bone mass in female mice.

Original language	English (US)
Pages (from-to)	129-136
Number of pages	8
Journal	Journal of Endocrinology
Volume	238
Issue number	2
DOIs	https://doi.org/10.1530/JOE-18-0183
State	Published - Aug 1 2018

Keywords

Bone loss
Estrogen
Estrogen receptor alpha
T lymphocytes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

Access to Document

10.1530/JOE-18-0183

Cite this

Gustafsson, K. L., Nilsson, K. H., Farman, H. H., Andersson, A., Lionikaite, V., Henning, P., Wu, J., Windahl, S. H., Islander, U., Movérare-Skrtic, S., Sjögren, K., Carlsten, H., Gustafsson, J., Ohlsson, C., & Lagerquist, M. K. (2018). ERα expression in T lymphocytes is dispensable for estrogenic effects in bone. Journal of Endocrinology, 238(2), 129-136. https://doi.org/10.1530/JOE-18-0183

Gustafsson, KL, Nilsson, KH, Farman, HH, Andersson, A, Lionikaite, V, Henning, P, Wu, J, Windahl, SH, Islander, U, Movérare-Skrtic, S, Sjögren, K, Carlsten, H, Gustafsson, J, Ohlsson, C & Lagerquist, MK 2018, 'ERα expression in T lymphocytes is dispensable for estrogenic effects in bone', Journal of Endocrinology, vol. 238, no. 2, pp. 129-136. https://doi.org/10.1530/JOE-18-0183

@article{5a97fead72f641eabc706af7d52ff143,

title = "ERα expression in T lymphocytes is dispensable for estrogenic effects in bone",

abstract = "Estrogen treatment has positive effects on the skeleton, and we have shown that estrogen receptor alpha (ERα) expression in cells of hematopoietic origin contributes to a normal estrogen treatment response in bone tissue. T lymphocytes are implicated in the estrogenic regulation of bone mass, but it is not known whether T lymphocytes are direct estrogen target cells. Therefore, the aim of this study was to determine the importance of ERα expression in T lymphocytes for the estrogenic regulation of the skeleton using female mice lacking ERα expression specifically in T lymphocytes (Lck-ERα-/-) and ERαflox/flox littermate (control) mice. Deletion of ERα expression in T lymphocytes did not affect bone mineral density (BMD) in sham-operated Lck-ERα-/- compared to control mice, and ovariectomy (ovx) resulted in a similar decrease in BMD in control and Lck-ERα-/- mice compared to sham-operated mice. Furthermore, estrogen treatment of ovx Lck-ERα-/- led to an increased BMD that was indistinguishable from the increase seen after estrogen treatment of ovx control mice. Detailed analysis of both the appendicular (femur) and axial (vertebrae) skeleton showed that both trabecular and cortical bone parameters responded to a similar extent regardless of the presence of ERα in T lymphocytes. In conclusion, ERα expression in T lymphocytes is dispensable for normal estrogenic regulation of bone mass in female mice.",

keywords = "Bone loss, Estrogen, Estrogen receptor alpha, T lymphocytes",

author = "Gustafsson, {K. L.} and Nilsson, {K. H.} and Farman, {H. H.} and A. Andersson and V. Lionikaite and P. Henning and J. Wu and Windahl, {S. H.} and U. Islander and S. Mov{\'e}rare-Skrtic and K. Sj{\"o}gren and H. Carlsten and J. Gustafsson and C. Ohlsson and Lagerquist, {M. K.}",

note = "Funding Information: The Swedish Research Council (grant number 2017-01286); the Swedish Foundation for Strategic Research; the ALF/LUA research grant from the Sahlgrenska University Hospital (grant number ALFGBG-721581); the Gustaf V 80-year fund (grant number FAI-2016-0286); the Swedish Rheumatism Association (grant number R-754891); the Lundberg Foundation (grant number 2017-0076); the Torsten and Ragnar S{\"o}derberg{\textquoteright}s Foundations (grant number M133/12); the Knut and Alice Wallenberg Foundation (grant number KAW 2015.0317) and the Novo Nordisk Foundation (grant number NNF17OC0026844). J A G is thankful to the Robert A. Welch Foundation for a grant (E-0004). Publisher Copyright: {\textcopyright} 2018 The authors Published by Bioscientifica Ltd.",

year = "2018",

month = aug,

day = "1",

doi = "10.1530/JOE-18-0183",

language = "English (US)",

volume = "238",

pages = "129--136",

journal = "Journal of Endocrinology",

issn = "0022-0795",

publisher = "Society for Endocrinology",

number = "2",

}

TY - JOUR

T1 - ERα expression in T lymphocytes is dispensable for estrogenic effects in bone

AU - Gustafsson, K. L.

AU - Nilsson, K. H.

AU - Farman, H. H.

AU - Andersson, A.

AU - Lionikaite, V.

AU - Henning, P.

AU - Wu, J.

AU - Windahl, S. H.

AU - Islander, U.

AU - Movérare-Skrtic, S.

AU - Sjögren, K.

AU - Carlsten, H.

AU - Gustafsson, J.

AU - Ohlsson, C.

AU - Lagerquist, M. K.

N1 - Funding Information: The Swedish Research Council (grant number 2017-01286); the Swedish Foundation for Strategic Research; the ALF/LUA research grant from the Sahlgrenska University Hospital (grant number ALFGBG-721581); the Gustaf V 80-year fund (grant number FAI-2016-0286); the Swedish Rheumatism Association (grant number R-754891); the Lundberg Foundation (grant number 2017-0076); the Torsten and Ragnar Söderberg’s Foundations (grant number M133/12); the Knut and Alice Wallenberg Foundation (grant number KAW 2015.0317) and the Novo Nordisk Foundation (grant number NNF17OC0026844). J A G is thankful to the Robert A. Welch Foundation for a grant (E-0004). Publisher Copyright: © 2018 The authors Published by Bioscientifica Ltd.

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Estrogen treatment has positive effects on the skeleton, and we have shown that estrogen receptor alpha (ERα) expression in cells of hematopoietic origin contributes to a normal estrogen treatment response in bone tissue. T lymphocytes are implicated in the estrogenic regulation of bone mass, but it is not known whether T lymphocytes are direct estrogen target cells. Therefore, the aim of this study was to determine the importance of ERα expression in T lymphocytes for the estrogenic regulation of the skeleton using female mice lacking ERα expression specifically in T lymphocytes (Lck-ERα-/-) and ERαflox/flox littermate (control) mice. Deletion of ERα expression in T lymphocytes did not affect bone mineral density (BMD) in sham-operated Lck-ERα-/- compared to control mice, and ovariectomy (ovx) resulted in a similar decrease in BMD in control and Lck-ERα-/- mice compared to sham-operated mice. Furthermore, estrogen treatment of ovx Lck-ERα-/- led to an increased BMD that was indistinguishable from the increase seen after estrogen treatment of ovx control mice. Detailed analysis of both the appendicular (femur) and axial (vertebrae) skeleton showed that both trabecular and cortical bone parameters responded to a similar extent regardless of the presence of ERα in T lymphocytes. In conclusion, ERα expression in T lymphocytes is dispensable for normal estrogenic regulation of bone mass in female mice.

AB - Estrogen treatment has positive effects on the skeleton, and we have shown that estrogen receptor alpha (ERα) expression in cells of hematopoietic origin contributes to a normal estrogen treatment response in bone tissue. T lymphocytes are implicated in the estrogenic regulation of bone mass, but it is not known whether T lymphocytes are direct estrogen target cells. Therefore, the aim of this study was to determine the importance of ERα expression in T lymphocytes for the estrogenic regulation of the skeleton using female mice lacking ERα expression specifically in T lymphocytes (Lck-ERα-/-) and ERαflox/flox littermate (control) mice. Deletion of ERα expression in T lymphocytes did not affect bone mineral density (BMD) in sham-operated Lck-ERα-/- compared to control mice, and ovariectomy (ovx) resulted in a similar decrease in BMD in control and Lck-ERα-/- mice compared to sham-operated mice. Furthermore, estrogen treatment of ovx Lck-ERα-/- led to an increased BMD that was indistinguishable from the increase seen after estrogen treatment of ovx control mice. Detailed analysis of both the appendicular (femur) and axial (vertebrae) skeleton showed that both trabecular and cortical bone parameters responded to a similar extent regardless of the presence of ERα in T lymphocytes. In conclusion, ERα expression in T lymphocytes is dispensable for normal estrogenic regulation of bone mass in female mice.

KW - Bone loss

KW - Estrogen

KW - Estrogen receptor alpha

KW - T lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=85049441409&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049441409&partnerID=8YFLogxK

U2 - 10.1530/JOE-18-0183

DO - 10.1530/JOE-18-0183

M3 - Article

C2 - 29848607

AN - SCOPUS:85049441409

SN - 0022-0795

VL - 238

SP - 129

EP - 136

JO - Journal of Endocrinology

JF - Journal of Endocrinology

IS - 2

ER -

ERα expression in T lymphocytes is dispensable for estrogenic effects in bone

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this