Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System

Research output: Contribution to journalArticle

Yi-Lan Weng, Xu Wang, Ran An, Jessica Cassin, Caroline Vissers, Yuanyuan Liu, Yajing Liu, Tianlei Xu, Xinyuan Wang, Samuel Zheng Hao Wong, Jessica Joseph, Louis C Dore, Qiang Dong, Wei Zheng, Peng Jin, Hao Wu, Bin Shen, Xiaoxi Zhuang, Chuan He, Kai Liu & 2 others Hongjun Song, Guo-Li Ming

N6-methyladenosine (m6A) affects multiple aspects of mRNA metabolism and regulates developmental transitions by promoting mRNA decay. Little is known about the role of m6A in the adult mammalian nervous system. Here we report that sciatic nerve lesion elevates levels of m6A-tagged transcripts encoding many regeneration-associated genes and protein translation machinery components in the adult mouse dorsal root ganglion (DRG). Single-base resolution m6A-CLIP mapping further reveals a dynamic m6A landscape in the adult DRG upon injury. Loss of either m6A methyltransferase complex component Mettl14 or m6A-binding protein Ythdf1 globally attenuates injury-induced protein translation in adult DRGs and reduces functional axon regeneration in the peripheral nervous system in vivo. Furthermore, Pten deletion-induced axon regeneration of retinal ganglion neurons in the adult central nervous system is attenuated upon Mettl14 knockdown. Our study reveals a critical epitranscriptomic mechanism in promoting injury-induced protein synthesis and axon regeneration in the adult mammalian nervous system.

Original languageEnglish (US)
Pages (from-to)313-325.e6
JournalNeuron
Volume97
Issue number2
DOIs
StatePublished - Jan 17 2018

PMID: 29346752

PMCID: PMC5777326

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Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System. / Weng, Yi-Lan; Wang, Xu; An, Ran; Cassin, Jessica; Vissers, Caroline; Liu, Yuanyuan; Liu, Yajing; Xu, Tianlei; Wang, Xinyuan; Wong, Samuel Zheng Hao; Joseph, Jessica; Dore, Louis C; Dong, Qiang; Zheng, Wei; Jin, Peng; Wu, Hao; Shen, Bin; Zhuang, Xiaoxi; He, Chuan; Liu, Kai; Song, Hongjun; Ming, Guo-Li.

In: Neuron, Vol. 97, No. 2, 17.01.2018, p. 313-325.e6.

Research output: Contribution to journalArticle

Harvard

Weng, Y-L, Wang, X, An, R, Cassin, J, Vissers, C, Liu, Y, Liu, Y, Xu, T, Wang, X, Wong, SZH, Joseph, J, Dore, LC, Dong, Q, Zheng, W, Jin, P, Wu, H, Shen, B, Zhuang, X, He, C, Liu, K, Song, H & Ming, G-L 2018, 'Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System' Neuron, vol. 97, no. 2, pp. 313-325.e6. https://doi.org/10.1016/j.neuron.2017.12.036

APA

Weng, Y-L., Wang, X., An, R., Cassin, J., Vissers, C., Liu, Y., ... Ming, G-L. (2018). Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System. Neuron, 97(2), 313-325.e6. https://doi.org/10.1016/j.neuron.2017.12.036

Vancouver

Weng Y-L, Wang X, An R, Cassin J, Vissers C, Liu Y et al. Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System. Neuron. 2018 Jan 17;97(2):313-325.e6. https://doi.org/10.1016/j.neuron.2017.12.036

Author

Weng, Yi-Lan ; Wang, Xu ; An, Ran ; Cassin, Jessica ; Vissers, Caroline ; Liu, Yuanyuan ; Liu, Yajing ; Xu, Tianlei ; Wang, Xinyuan ; Wong, Samuel Zheng Hao ; Joseph, Jessica ; Dore, Louis C ; Dong, Qiang ; Zheng, Wei ; Jin, Peng ; Wu, Hao ; Shen, Bin ; Zhuang, Xiaoxi ; He, Chuan ; Liu, Kai ; Song, Hongjun ; Ming, Guo-Li. / Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System. In: Neuron. 2018 ; Vol. 97, No. 2. pp. 313-325.e6.

BibTeX

@article{4884d7bf0da04f1195ec386396836cd7,
title = "Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System",
abstract = "N6-methyladenosine (m6A) affects multiple aspects of mRNA metabolism and regulates developmental transitions by promoting mRNA decay. Little is known about the role of m6A in the adult mammalian nervous system. Here we report that sciatic nerve lesion elevates levels of m6A-tagged transcripts encoding many regeneration-associated genes and protein translation machinery components in the adult mouse dorsal root ganglion (DRG). Single-base resolution m6A-CLIP mapping further reveals a dynamic m6A landscape in the adult DRG upon injury. Loss of either m6A methyltransferase complex component Mettl14 or m6A-binding protein Ythdf1 globally attenuates injury-induced protein translation in adult DRGs and reduces functional axon regeneration in the peripheral nervous system in vivo. Furthermore, Pten deletion-induced axon regeneration of retinal ganglion neurons in the adult central nervous system is attenuated upon Mettl14 knockdown. Our study reveals a critical epitranscriptomic mechanism in promoting injury-induced protein synthesis and axon regeneration in the adult mammalian nervous system.",
author = "Yi-Lan Weng and Xu Wang and Ran An and Jessica Cassin and Caroline Vissers and Yuanyuan Liu and Yajing Liu and Tianlei Xu and Xinyuan Wang and Wong, {Samuel Zheng Hao} and Jessica Joseph and Dore, {Louis C} and Qiang Dong and Wei Zheng and Peng Jin and Hao Wu and Bin Shen and Xiaoxi Zhuang and Chuan He and Kai Liu and Hongjun Song and Guo-Li Ming",
note = "Copyright {\circledC} 2017 Elsevier Inc. All rights reserved.",
year = "2018",
month = "1",
day = "17",
doi = "10.1016/j.neuron.2017.12.036",
language = "English (US)",
volume = "97",
pages = "313--325.e6",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System

AU - Weng, Yi-Lan

AU - Wang, Xu

AU - An, Ran

AU - Cassin, Jessica

AU - Vissers, Caroline

AU - Liu, Yuanyuan

AU - Liu, Yajing

AU - Xu, Tianlei

AU - Wang, Xinyuan

AU - Wong, Samuel Zheng Hao

AU - Joseph, Jessica

AU - Dore, Louis C

AU - Dong, Qiang

AU - Zheng, Wei

AU - Jin, Peng

AU - Wu, Hao

AU - Shen, Bin

AU - Zhuang, Xiaoxi

AU - He, Chuan

AU - Liu, Kai

AU - Song, Hongjun

AU - Ming, Guo-Li

N1 - Copyright © 2017 Elsevier Inc. All rights reserved.

PY - 2018/1/17

Y1 - 2018/1/17

N2 - N6-methyladenosine (m6A) affects multiple aspects of mRNA metabolism and regulates developmental transitions by promoting mRNA decay. Little is known about the role of m6A in the adult mammalian nervous system. Here we report that sciatic nerve lesion elevates levels of m6A-tagged transcripts encoding many regeneration-associated genes and protein translation machinery components in the adult mouse dorsal root ganglion (DRG). Single-base resolution m6A-CLIP mapping further reveals a dynamic m6A landscape in the adult DRG upon injury. Loss of either m6A methyltransferase complex component Mettl14 or m6A-binding protein Ythdf1 globally attenuates injury-induced protein translation in adult DRGs and reduces functional axon regeneration in the peripheral nervous system in vivo. Furthermore, Pten deletion-induced axon regeneration of retinal ganglion neurons in the adult central nervous system is attenuated upon Mettl14 knockdown. Our study reveals a critical epitranscriptomic mechanism in promoting injury-induced protein synthesis and axon regeneration in the adult mammalian nervous system.

AB - N6-methyladenosine (m6A) affects multiple aspects of mRNA metabolism and regulates developmental transitions by promoting mRNA decay. Little is known about the role of m6A in the adult mammalian nervous system. Here we report that sciatic nerve lesion elevates levels of m6A-tagged transcripts encoding many regeneration-associated genes and protein translation machinery components in the adult mouse dorsal root ganglion (DRG). Single-base resolution m6A-CLIP mapping further reveals a dynamic m6A landscape in the adult DRG upon injury. Loss of either m6A methyltransferase complex component Mettl14 or m6A-binding protein Ythdf1 globally attenuates injury-induced protein translation in adult DRGs and reduces functional axon regeneration in the peripheral nervous system in vivo. Furthermore, Pten deletion-induced axon regeneration of retinal ganglion neurons in the adult central nervous system is attenuated upon Mettl14 knockdown. Our study reveals a critical epitranscriptomic mechanism in promoting injury-induced protein synthesis and axon regeneration in the adult mammalian nervous system.

U2 - 10.1016/j.neuron.2017.12.036

DO - 10.1016/j.neuron.2017.12.036

M3 - Article

VL - 97

SP - 313-325.e6

JO - Neuron

T2 - Neuron

JF - Neuron

SN - 0896-6273

IS - 2

ER -

ID: 41875050