Enzyme biotransducers formed from conductive electroactive polymers

Guneet Bedi, Christian Kotanen, Olukayode Karunwi, Amanda Nguyen, Ferhat Bayram, Brian Hudson, Yu Zhao, Anthony Guiseppi-Elie

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Three types of biotransducer devices were studied: an enzyme Field Effect Transistor (Enz-FET), an interdigitated microsensor electrode (IME) array for impedimetric detection, and a microdisc electrode array for amperometric detection. Each device type was microlithographically fabricated from a pattern of sputter deposited metal (10 nm Ti/W 100 nm Pt or Au) on an insulating substrate that was either glass or oxidized p-type silicon. Insulating regions were fashioned from Si3N4 and the gate dielectric of the FET was a SiO2 layer of 100 nm thick spin-on glass. For each biotransducer device type, the enzyme glucose oxidase (GOx) was immobilized using a process of electropolymerization of pyrrole (0.8 V vs. Ag/AgCl) in the presences of glucose oxidase (1 mg/ml) in deionized water to achieve a total of 100 mC/cm2. The dose-response characteristics of each of the biotransducers were tested in muta-rotated glucose solutions (PBS 7.2) at RT that ranged from 0.0 mM to 20 mM. The sensitivity (m=slope), linear dynamic range and limits of detection [3(SDblank/m)] were calculated from replicate measures of the dose response curve.

Original languageEnglish (US)
Title of host publicationIEEE SENSORS 2013 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Print)9781467346405
DOIs
StatePublished - Jan 1 2013
Event12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States
Duration: Nov 4 2013Nov 6 2013

Other

Other12th IEEE SENSORS 2013 Conference
CountryUnited States
CityBaltimore, MD
Period11/4/1311/6/13

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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