Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: A new approach to magnetic sensing

Arati G. Kolhatkar; Andrew C. Jamison; Ivan Nekrashevich; Katerina Kourentzi; Dmitri Litvinov; Audrius Brazdeikis; Richard C. Willson; T. Randall Lee

doi:10.1039/c6an00709k

Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: A new approach to magnetic sensing

Arati G. Kolhatkar, Andrew C. Jamison, Ivan Nekrashevich, Katerina Kourentzi, Dmitri Litvinov, Audrius Brazdeikis, Richard C. Willson, T. Randall Lee

Houston Methodist

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Magnetic sensing utilizes the detection of biomolecule-conjugated magnetic nanoparticles (MNPs). Our new strategy offers a novel approach to magnetic sensing where in situ conversion produces a "loss of signal" in the sensing device. This report demonstrates the enzymatic conversion of Fe₃O₄ MNPs to a non-magnetic precipitate via reduction by l-ascorbic acid generated by the action of alkaline phosphatase.

Original language	English (US)
Pages (from-to)	5246-5251
Number of pages	6
Journal	Analyst
Volume	141
Issue number	18
DOIs	https://doi.org/10.1039/c6an00709k
State	Published - Sep 21 2016

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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title = "Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: A new approach to magnetic sensing",

abstract = "Magnetic sensing utilizes the detection of biomolecule-conjugated magnetic nanoparticles (MNPs). Our new strategy offers a novel approach to magnetic sensing where in situ conversion produces a {"}loss of signal{"} in the sensing device. This report demonstrates the enzymatic conversion of Fe3O4 MNPs to a non-magnetic precipitate via reduction by l-ascorbic acid generated by the action of alkaline phosphatase.",

author = "Kolhatkar, {Arati G.} and Jamison, {Andrew C.} and Ivan Nekrashevich and Katerina Kourentzi and Dmitri Litvinov and Audrius Brazdeikis and Willson, {Richard C.} and Lee, {T. Randall}",

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T2 - A new approach to magnetic sensing

AU - Kolhatkar, Arati G.

AU - Jamison, Andrew C.

AU - Nekrashevich, Ivan

AU - Kourentzi, Katerina

AU - Litvinov, Dmitri

AU - Brazdeikis, Audrius

AU - Willson, Richard C.

AU - Lee, T. Randall

PY - 2016/9/21

Y1 - 2016/9/21

N2 - Magnetic sensing utilizes the detection of biomolecule-conjugated magnetic nanoparticles (MNPs). Our new strategy offers a novel approach to magnetic sensing where in situ conversion produces a "loss of signal" in the sensing device. This report demonstrates the enzymatic conversion of Fe3O4 MNPs to a non-magnetic precipitate via reduction by l-ascorbic acid generated by the action of alkaline phosphatase.

AB - Magnetic sensing utilizes the detection of biomolecule-conjugated magnetic nanoparticles (MNPs). Our new strategy offers a novel approach to magnetic sensing where in situ conversion produces a "loss of signal" in the sensing device. This report demonstrates the enzymatic conversion of Fe3O4 MNPs to a non-magnetic precipitate via reduction by l-ascorbic acid generated by the action of alkaline phosphatase.

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Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: A new approach to magnetic sensing

Abstract

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