Enterococcus faecalis from newborn babies regulate endogenous PPARγ activity and IL-10 levels in colonic epithelial cells

Alexandra Are, Linda Aronsson, Shugui Wang, Gediminas Greicius, Kun Lee Yuan, Jan Åke Gustafsson, Sven Pettersson, Velmurugesan Arulampalam

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

The postembryonic development of the gastrointestinal tract is subject to regulation by the colonizing microbiota. This maturation process requires the commensal bacteria to cross-talk with host cells by way of recognizing receptors and inducing signaling pathways to activate transcription factors such as the nuclear receptors. Here, we show that in colonic cell lines and in primary colonic cells, Enterococcus faecalis isolated from newborn babies possess the ability to regulate peroxisome proliferator-activated receptor-γ1 (PPARγ1) activity through phosphorylation. This results in elevated DNA binding and transcriptional activation of downstream target genes, including IL-10, a cytokine known to modulate innate immune function. Furthermore, phosphorylation appears tightly regulated as phospho-PPARγ1 becomes an immediate substrate for degradation possibly to curtail any extended transactivation. The involvement of PPARγ1 in a myriad of physiological processes further confirms that microflora-driven regulation might be important for a number of homeostatic strategies in the gut.

Original languageEnglish (US)
Pages (from-to)1943-1948
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number6
DOIs
StatePublished - Feb 12 2008

Keywords

  • Microbe-host interaction
  • Nuclear receptors
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • General

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