Enhancement of transesterification-catalyzing capability of bio-imprinted tannase in organic solvents by cryogenic protection and immobilization

Guangjun Nie, Zhen Chen, Zhiming Zheng, Wei Jin, Guohong Gong, Li Wang, Wenjin Yue

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Improvement of transesterification-catalyzing capability of bio-imprinted tannase is a crucial question of whether to be efficiently utilized in organic media. As for biotransformation of tannic acid to propyl gallate, bio-imprinting technique can dramatically enhance the transesterification-catalyzing capability of tannase. In this work, both cryogenic protection and immobilization were utilized to further improve its apparent catalytic capability in organic media. The results show that Triton-X-100, mannose, and magnesium ion all have a positive effect on cryogenic protection of the tannase. Particularly, combinational application of the three cryoprotectants increases its catalytic performance by 2.7-fold factor. Also, immobilization further elevates its catalytic capability by 2.1 folds. Noteworthily, the coupling application of immobilization and cryo-protection can cause the conversion rate of substrate of the bio-imprinted tannase to increase to a promising 70%. Consequently, it will be helpful to fully utilize tannase in organic phase.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Molecular Catalysis B: Enzymatic
Volume94
DOIs
StatePublished - Jan 1 2013

Keywords

  • Bio-imprinting
  • Cryogenic protection
  • Immobilization
  • Propyl gallate
  • Tannase
  • Tannic acid

ASJC Scopus subject areas

  • Biochemistry
  • Bioengineering
  • Catalysis
  • Process Chemistry and Technology

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