Enhancement of the AMPLICOR enterovirus PCR test with a coprecipitant

E. William Taggart, Carrie L. Byington, David R. Hillyard, John E. Robison, Karen C. Carroll

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The incorporation of a commercially available coprecipitant into the AMPLICOR enterovirus PCR test specimen preparation enhanced the sensitivity and reproducibility of this assay. Fifty-five previously tested archived cerebrospinal fluids (CSF) specimens were tested in a blind study in duplicate with and without Pellet Paint coprecipitant (Novagen, Inc., Madison, Wis.). Of these specimens, 26 had previously been determined to be positive and 29 had previously been determined to be negative. All previously positive CSF specimens were positive when Pellet Paint was used and only 18 were positive without Pellet Paint. No previously negative specimens were positive on repeat testing with or without Pellet Paint. The background signal was not affected by the addition of Pellet Paint. These data support the utility of a coprecipitant in minimizing false-negative results.

Original languageEnglish (US)
Pages (from-to)3408-3409
Number of pages2
JournalJournal of Clinical Microbiology
Volume36
Issue number11
DOIs
StatePublished - Nov 1998

ASJC Scopus subject areas

  • Microbiology (medical)

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