Enhanced protein affinity and selectivity of clustered-charge anion-exchange adsorbents

Joseph Y. Fu, Sindhu Balan, Ajish Potty, Van Nguyen, Richard C. Willson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In this work, we examined the possibility of improving ion-exchange adsorbent performance by nanoscale structuring of ligands into clusters of fixed size rather than a random distribution of individual charges. The calcium-depleted form of the protein α-lactalbumin, which displays a cluster of acidic amino acid residues, showed enhanced adsorption affinity and capacity on clustered-charge pentalysinamide and pentaargininamide adsorbents as compared to single-charge lysinamide and argininamide adsorbents of matched total charge. Two differently charge-clustered mutants of rat microsomal cytochrome b5, E11Q and E44Q, with the same total charge also were well differentiated by clustered-charge adsorbents. Thus, an organized rather than random distribution of charges may produce adsorbents with higher capacity and selectivity, especially for biomolecules with inherent charge clustering.

Original languageEnglish (US)
Pages (from-to)9060-9065
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number23
DOIs
StatePublished - Dec 1 2007

ASJC Scopus subject areas

  • Analytical Chemistry

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