Enhanced lymph node retention of subcutaneously injected IgG1-PEG2000-liposomes through pentameric IgM antibody-mediated vesicular aggregation

S. Moein Moghimi, Majid Moghimi

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

An efficient strategy for enhancing the lymph node deposition of rapidly drained liposomes from the interstitial injection site is described. Subcutaneously injected small-sized immuno-poly(ethyleneglycol)-liposomes (immuno-PEG-liposomes), containing 10 mol% mPEG350-phospholipid and 1 mol% PEG2000-phospholipid in their bilayer and where IgG1 is coupled to the distal end of PEG2000, not only drain rapidly from the interstitial spaces into the initial lymphatic system, but also accumulate efficiently among the lymph nodes draining the region when compared with non-PEG-bearing immunoliposomes where IgG is directly coupled to the phospholipid. Liposome deposition among the draining lymph nodes, however, was further enhanced dramatically following an adjacent subcutaneous injection of a pentameric IgM against the surface attached IgG molecules (IgM:IgG, 10:1) without compromising vesicle drainage from the interstitium. This is suggested to arise either as a result of formation of large immuno-aggregates within the lymphatic vessels with subsequent transport to and trapping among the regional lymph nodes and/or following IgM binding to Fc receptors of the lymph node sinus macrophages forming a platform for subsequent trapping of drained IgG-coupled liposomes. This lymph node targeting approach may be amenable for the design and surface engineering of any rapidly drained nanoparticulate system bearing peptides and proteins that can be aggregated with a desired monoclonal pentameric IgM.

Original languageEnglish (US)
Pages (from-to)51-55
Number of pages5
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1778
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • IgM
  • Immunoliposome
  • Lymph node
  • Lymphatic system
  • Macrophage
  • Targeting

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Fingerprint Dive into the research topics of 'Enhanced lymph node retention of subcutaneously injected IgG1-PEG<sub>2000</sub>-liposomes through pentameric IgM antibody-mediated vesicular aggregation'. Together they form a unique fingerprint.

Cite this