Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte

Shutao Guo, Yuanyu Huang, Qiao Jiang, Yun Sun, Liandong Deng, Zicai Liang, Quan Du, Jinfeng Xing, Yuliang Zhao, Paul C. Wang, Anjie Dong, Xing Jie Liang

Research output: Contribution to journalArticle

311 Scopus citations

Abstract

Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence of the charge-reversal property of functional gold nanoparticles. The expression efficiency of enhanced green fluorescent protein (EGFP) was improved by adjuvant transfection with charge-reversal functional gold nanoparticles, which also had much lower toxicity to cell proliferation. Lamin A/C, an important nuclear envelope protein, was effectively silenced by lamin A/C-siRNA delivered by charge-reversal functional gold nanoparticles, whose knockdown efficiency was better than that of commercial Lipofectamine 2000. Confocal laser scanning microscopic images indicated that there was more cy5-siRNA distributed throughout the cytoplasm for cyanine 5-siRNA/polyethyleneimine/cis-aconitic anhydride-functionalized poly(allylamine)/ polyethyleneimine/11- mercaptoundecanoic acid-gold nanoparticle (cy5-siRNA/PEI/PAH-Cit/PEI/MUA-AuNP) complexes. These results demonstrate the feasibility of using charge-reversal functional gold nanoparticles as a means of improving the nucleic acid delivery efficiency.

Original languageEnglish (US)
Pages (from-to)5505-5511
Number of pages7
JournalACS Nano
Volume4
Issue number9
DOIs
StatePublished - Sep 28 2010

Keywords

  • charge-reversal polyelectrolyte
  • drug delivery
  • gold nanoparticles
  • layer-by-layer assembly
  • siRNA delivery

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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